Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3764621..3765162 Replicon chromosome
Accession NZ_CP096587
Organism Escherichia coli strain EC6622

Toxin (Protein)


Gene name yafQ Uniprot ID -
Locus tag M1B75_RS18500 Protein ID WP_217832132.1
Coordinates 3764884..3765162 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag M1B75_RS18495 Protein ID WP_000729704.1
Coordinates 3764621..3764881 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M1B75_RS18470 (3760404) 3760404..3761189 - 786 WP_000207575.1 putative lateral flagellar export/assembly protein LafU -
M1B75_RS18475 (3761161) 3761161..3762873 + 1713 Protein_3627 flagellar biosynthesis protein FlhA -
M1B75_RS18480 (3762978) 3762978..3763256 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
M1B75_RS18485 (3763249) 3763249..3763605 + 357 WP_001030483.1 type II toxin-antitoxin system HicB family antitoxin -
M1B75_RS18490 (3763662) 3763662..3764435 - 774 WP_000543895.1 C40 family peptidase -
M1B75_RS18495 (3764621) 3764621..3764881 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
M1B75_RS18500 (3764884) 3764884..3765162 + 279 WP_217832132.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
M1B75_RS18505 (3765318) 3765318..3766058 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
M1B75_RS18510 (3766029) 3766029..3766796 - 768 WP_000333380.1 class II glutamine amidotransferase -
M1B75_RS18515 (3767002) 3767002..3767580 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - gmhA/lpcA 3711869..3789393 77524


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10858.63 Da        Isoelectric Point: 9.8881

>T243201 WP_217832132.1 NZ_CP096587:3764884-3765162 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQDSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T243201 NZ_OW970422:c5224030-5223647 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT243201 WP_000729704.1 NZ_CP096587:3764621-3764881 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT243201 NZ_OW970422:c5224272-5224030 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References