Detailed information of TA system TA243014

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 110019..110746
• Replicon: plasmid pKP19-2796-1
• Accession: NZ_CP096261
• Organism: Klebsiella pneumoniae strain KP19-2796

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: M0M88_RS26505
• Protein ID: WP_011251285.1
• Coordinates: 110019..110330 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: M0M88_RS26510
• Protein ID: WP_011251286.1
• Coordinates: 110327..110746 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M0M88_RS26480 (M0M88_26480)	106785..106964	-	180	Protein_114	IS5/IS1182 family transposase	-
M0M88_RS26485 (M0M88_26485)	107004..108532	-	1529	Protein_115	IS66 family transposase	-
M0M88_RS26490 (M0M88_26490)	108581..108928	-	348	WP_004114612.1	IS66 family insertion sequence element accessory protein TnpB	-
M0M88_RS26495 (M0M88_26495)	108925..109263	-	339	WP_023288266.1	transposase	-
M0M88_RS26500 (M0M88_26500)	109377..109814	+	438	Protein_118	DDE-type integrase/transposase/recombinase	-
M0M88_RS26505 (M0M88_26505)	110019..110330	+	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
M0M88_RS26510 (M0M88_26510)	110327..110746	+	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
M0M88_RS26515 (M0M88_26515)	110893..111861	+	969	WP_077268729.1	IS5 family transposase	-
M0M88_RS26520 (M0M88_26520)	111930..112465	-	536	Protein_122	transposase	-
M0M88_RS26525 (M0M88_26525)	112990..114357	-	1368	WP_032437385.1	formimidoylglutamate deiminase	-
M0M88_RS26530 (M0M88_26530)	114460..115221	+	762	WP_017900871.1	histidine utilization repressor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aph(3')-Ia / mph(E) / msr(E) / armA / sul1 / blaDHA-1 / qnrB4 / aadA2 / cmlA1 / ant(3'')-Ia / sul3 / aac(3)-IVa / aph(4)-Ia / aph(6)-Id / aph(3'')-Ib	-	1..227518	227518
	-	inside	IScluster/Tn	-	-	79503..112324	32821

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T243014 WP_011251285.1 NZ_CP096261:110019-110330 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T243014 NZ_OW968288:c3786118-3785720 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT243014 WP_011251286.1 NZ_CP096261:110327-110746 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT243014 NZ_OW968288:c3786414-3786121 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1