Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3937801..3938398 Replicon chromosome
Accession NZ_CP096260
Organism Klebsiella pneumoniae strain KP19-2796

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag M0M88_RS19175 Protein ID WP_004142563.1
Coordinates 3938081..3938398 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag M0M88_RS19170 Protein ID WP_004142561.1
Coordinates 3937801..3938088 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M0M88_RS19140 (3933881) 3933881..3934129 + 249 WP_020325243.1 DUF1158 domain-containing protein -
M0M88_RS19145 (3934147) 3934147..3934488 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
M0M88_RS19150 (3934519) 3934519..3935634 - 1116 WP_020325248.1 MBL fold metallo-hydrolase -
M0M88_RS19155 (3935814) 3935814..3936395 + 582 WP_020325240.1 TetR/AcrR family transcriptional regulator -
M0M88_RS19160 (3936395) 3936395..3936763 + 369 WP_020325252.1 MmcQ/YjbR family DNA-binding protein -
M0M88_RS19165 (3936883) 3936883..3937536 + 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
M0M88_RS19170 (3937801) 3937801..3938088 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
M0M88_RS19175 (3938081) 3938081..3938398 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
M0M88_RS19180 (3938583) 3938583..3939626 - 1044 WP_020325241.1 DUF2157 domain-containing protein -
M0M88_RS19185 (3940296) 3940296..3941162 - 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
M0M88_RS19190 (3941271) 3941271..3942698 + 1428 WP_009308097.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T243007 WP_004142563.1 NZ_CP096260:c3938398-3938081 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T243007 NZ_OW968288:2006165-2006268 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT243007 WP_004142561.1 NZ_CP096260:c3938088-3937801 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT243007 NZ_OW968288:c2006306-2006035 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References