Detailed information of TA system TA024298

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4379578..4380180
• Replicon: chromosome
• Accession: NC_013353
• Organism: Escherichia coli O103:H2 str. 12009

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: ECO103_RS22615
• Protein ID: WP_000897305.1
• Coordinates: 4379869..4380180 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ECO103_RS22610
• Protein ID: WP_000356397.1
• Coordinates: 4379578..4379868 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECO103_RS22585	4374653..4376260	-	1608	WP_001222883.1	dipeptide ABC transporter substrate-binding protein DppA	-
ECO103_RS22590	4376903..4377112	-	210	Protein_4281	hypothetical protein	-
ECO103_RS22595	4377104..4378084	+	981	WP_000399648.1	IS110-like element IS621 family transposase	-
ECO103_RS22600	4378313..4378555	-	243	WP_001086388.1	hypothetical protein	-
ECO103_RS22605	4378774..4378992	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
ECO103_RS22610	4379578..4379868	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
ECO103_RS22615	4379869..4380180	-	312	WP_000897305.1	hypothetical protein	Toxin
ECO103_RS22620	4380409..4381317	+	909	WP_001162704.1	alpha/beta hydrolase	-
ECO103_RS22625	4381381..4382322	-	942	WP_001300148.1	fatty acid biosynthesis protein FabY	-
ECO103_RS22630	4382367..4382804	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
ECO103_RS22635	4382801..4383673	-	873	WP_000920762.1	virulence factor BrkB family protein	-
ECO103_RS22640	4383667..4384266	-	600	WP_001295269.1	glucose-1-phosphatase	-
ECO103_RS22645	4384365..4385150	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	4377104..4378084	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T24298 WP_000897305.1 NC_013353:c4380180-4379869 [Escherichia coli O103:H2 str. 12009]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T24298 NC_013353:c4380180-4379869 [Escherichia coli O103:H2 str. 12009]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT24298 WP_000356397.1 NC_013353:c4379868-4379578 [Escherichia coli O103:H2 str. 12009]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT24298 NC_013353:c4379868-4379578 [Escherichia coli O103:H2 str. 12009]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7