Detailed information of TA system TA242951

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 60089..60816
• Replicon: plasmid pVirDD02201
• Accession: NZ_CP096239
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain DD02201

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: M0536_RS26975
• Protein ID: WP_011251285.1
• Coordinates: 60505..60816 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: M0536_RS26970
• Protein ID: WP_011251286.1
• Coordinates: 60089..60508 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M0536_RS26940 (M0536_26900)	55257..55727	-	471	WP_048333570.1	hypothetical protein	-
M0536_RS26945 (M0536_26905)	56040..56675	-	636	WP_223171879.1	hypothetical protein	-
M0536_RS26950 (M0536_26910)	57094..57714	+	621	WP_223175004.1	conjugative transfer protein MobI(A/C)	-
M0536_RS26955 (M0536_26915)	57735..58523	+	789	WP_040217257.1	hypothetical protein	-
M0536_RS26960 (M0536_26920)	58537..58902	+	366	WP_048333448.1	hypothetical protein	-
M0536_RS26965 (M0536_26925)	58974..59942	-	969	WP_074428168.1	IS5 family transposase	-
M0536_RS26970 (M0536_26930)	60089..60508	-	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
M0536_RS26975 (M0536_26935)	60505..60816	-	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
M0536_RS26980 (M0536_26940)	61021..61458	-	438	Protein_60	DDE-type integrase/transposase/recombinase	-
M0536_RS26985 (M0536_26945)	61593..62290	+	698	WP_223175001.1	IS1-like element IS1A family transposase	-
M0536_RS26990 (M0536_26950)	63676..64326	-	651	WP_068893702.1	DUF1173 family protein	-
M0536_RS26995 (M0536_26955)	64359..64625	-	267	WP_223175074.1	DUF1173 family protein	-
M0536_RS27000 (M0536_26960)	64803..65297	+	495	WP_004212794.1	thermonuclease family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroB / iroC / iroD / iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2	1..234915	234915
	-	inside	IScluster/Tn	-	-	49968..62290	12322

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T242951 WP_011251285.1 NZ_CP096239:c60816-60505 [Klebsiella pneumoniae subsp. pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T242951 NZ_OW968124:2293494-2293781 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT242951 WP_011251286.1 NZ_CP096239:c60508-60089 [Klebsiella pneumoniae subsp. pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT242951 NZ_OW968124:2293255-2293494 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1