Detailed information of TA system TA242931

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 58479..59206
• Replicon: plasmid pVirDD02357
• Accession: NZ_CP096235
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain DD02357

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: M0537_RS26590
• Protein ID: WP_011251285.1
• Coordinates: 58895..59206 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: M0537_RS26585
• Protein ID: WP_011251286.1
• Coordinates: 58479..58898 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M0537_RS26555 (M0537_26525)	53647..54117	-	471	WP_048333570.1	hypothetical protein	-
M0537_RS26560 (M0537_26530)	54430..55065	-	636	WP_223171879.1	hypothetical protein	-
M0537_RS26565 (M0537_26535)	55484..56104	+	621	WP_223175004.1	conjugative transfer protein MobI(A/C)	-
M0537_RS26570 (M0537_26540)	56125..56913	+	789	WP_040217257.1	hypothetical protein	-
M0537_RS26575 (M0537_26545)	56927..57292	+	366	WP_048333448.1	hypothetical protein	-
M0537_RS26580 (M0537_26550)	57364..58332	-	969	WP_086642568.1	IS5 family transposase	-
M0537_RS26585 (M0537_26555)	58479..58898	-	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
M0537_RS26590 (M0537_26560)	58895..59206	-	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
M0537_RS26595 (M0537_26565)	59411..59848	-	438	Protein_60	DDE-type integrase/transposase/recombinase	-
M0537_RS26600 (M0537_26570)	59983..60680	+	698	WP_223175001.1	IS1-like element IS1A family transposase	-
M0537_RS26605 (M0537_26575)	60682..61131	+	450	WP_011251283.1	thioredoxin fold domain-containing protein	-
M0537_RS26610 (M0537_26580)	61148..61459	+	312	WP_011251282.1	hypothetical protein	-
M0537_RS26615 (M0537_26585)	61473..61814	+	342	WP_011251281.1	hypothetical protein	-
M0537_RS26620 (M0537_26590)	61874..62830	+	957	WP_011251280.1	DsbA family protein	-
M0537_RS26625 (M0537_26595)	62947..63522	+	576	WP_074424927.1	hypothetical protein	-
M0537_RS26630 (M0537_26600)	63630..64145	+	516	WP_041937820.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroB / iroC / iroD / iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA	1..229626	229626
	-	inside	IScluster/Tn	-	-	52657..60680	8023

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T242931 WP_011251285.1 NZ_CP096235:c59206-58895 [Klebsiella pneumoniae subsp. pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T242931 NZ_OW968111:2365044-2365874 [Escherichia coli]
ATGCCTAAACTTGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCCAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CAGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCTAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTAACGTTATTACCCGAAAA
TGAAACCATAACGCGCCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGGCTTGAAGATCGTTATGATTTTATGAAAT
TCCAGGTCTTCCAGTGGTTGATTGGCGCAACGGATGGCCATGCAAAAAACTTCTCCGTATTTATTCAGGCTGGCGGCAGT
TATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTCCTTGGCGGTACGGGAATACACATCAGCGATCTCAA
ACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATTTTTTGGCGACAG
CAAAGGTGCTGAGATTCCCGGAAGTGCAGATGCATGAAATCCTAAGTGACTTTGCCAGAATGATTCCGGCAGCACTGGAT
AACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAACCAATGTGTTGAGGTTGCATGG
ACGGTTAAGCCGAGAATACGGTAGTAAGTGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT242931 WP_011251286.1 NZ_CP096235:c58898-58479 [Klebsiella pneumoniae subsp. pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT242931 NZ_OW968111:2364778-2365044 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTGGCGAATGCAATGAAACTGGTTCGCCAGCAAAATGGCTGGAC
GCAGAGCGAGCTGGCGAAAAAAATTGGCATTAAGCAGGCGACAATTTCCAATTTCGAAAACAACCCTGACAATACCACGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACACTATGCGACACGAAAAATGCCTCGCCAGAATCA
ACAGAACAGCAAGATCTGGAGTGGTAA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1