Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2499815..2500317 Replicon chromosome
Accession NC_013353
Organism Escherichia coli O103:H2 str. 12009

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag ECO103_RS13165 Protein ID WP_000767829.1
Coordinates 2499815..2500069 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag ECO103_RS13170 Protein ID WP_001259255.1
Coordinates 2500066..2500317 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECO103_RS13140 2494830..2495057 - 228 WP_000985280.1 sulfurtransferase TusA family protein -
ECO103_RS13145 2495071..2496129 - 1059 WP_000492346.1 transport protein YeeE -
ECO103_RS13150 2496308..2497666 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
ECO103_RS31575 2497656..2497718 - 63 WP_010723108.1 membrane protein YoeI -
ECO103_RS13155 2497933..2498862 - 930 WP_000803351.1 LysR family transcriptional regulator -
ECO103_RS13160 2498908..2499732 - 825 WP_000754737.1 SDR family oxidoreductase -
ECO103_RS13165 2499815..2500069 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
ECO103_RS13170 2500066..2500317 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
ECO103_RS29990 2500600..2500650 + 51 WP_001368802.1 his operon leader peptide -
ECO103_RS13175 2500796..2501695 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
ECO103_RS13180 2501701..2503005 + 1305 WP_001298841.1 histidinol dehydrogenase -
ECO103_RS13185 2503002..2504072 + 1071 WP_000108980.1 histidinol-phosphate transaminase -
ECO103_RS13190 2504072..2505139 + 1068 WP_000080085.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T24290 WP_000767829.1 NC_013353:c2500069-2499815 [Escherichia coli O103:H2 str. 12009]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T24290 NC_013353:c2500069-2499815 [Escherichia coli O103:H2 str. 12009]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT24290 WP_001259255.1 NC_013353:c2500317-2500066 [Escherichia coli O103:H2 str. 12009]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT24290 NC_013353:c2500317-2500066 [Escherichia coli O103:H2 str. 12009]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References