Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 326332..326918 Replicon chromosome
Accession NZ_CP096230
Organism Klebsiella pneumoniae subsp. pneumoniae strain H39

Toxin (Protein)


Gene name doc Uniprot ID A0A330WAT7
Locus tag M0538_RS01520 Protein ID WP_014906830.1
Coordinates 326550..326918 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID W9B1V1
Locus tag M0538_RS01515 Protein ID WP_004174006.1
Coordinates 326332..326553 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M0538_RS01495 (322489) 322489..323415 + 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
M0538_RS01500 (323412) 323412..324689 + 1278 WP_270104416.1 branched chain amino acid ABC transporter permease LivM -
M0538_RS01505 (324686) 324686..325453 + 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
M0538_RS01510 (325455) 325455..326168 + 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
M0538_RS01515 (326332) 326332..326553 + 222 WP_004174006.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
M0538_RS01520 (326550) 326550..326918 + 369 WP_014906830.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
M0538_RS01525 (327191) 327191..328507 + 1317 WP_004174008.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
M0538_RS01530 (328614) 328614..329501 + 888 WP_014906831.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
M0538_RS01535 (329498) 329498..330343 + 846 WP_004145129.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
M0538_RS01540 (330345) 330345..331415 + 1071 WP_004150074.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 323412..332152 8740


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(28-86)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13537.95 Da        Isoelectric Point: 8.6410

>T242894 WP_014906830.1 NZ_CP096230:326550-326918 [Klebsiella pneumoniae subsp. pneumoniae]
MTLQIISAEEIILFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T242894 NZ_OW967976:c69544-69395 [Escherichia coli]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAGCTGAATATTCACAGAGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8151.15 Da        Isoelectric Point: 4.7769

>AT242894 WP_004174006.1 NZ_CP096230:326332-326553 [Klebsiella pneumoniae subsp. pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT242894 NZ_OW967976:69588-69649 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A330WAT7

Antitoxin

Source ID Structure
AlphaFold DB A0A5E5YJY7

References