Detailed information of TA system TA242880

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 274770..275305
• Replicon: plasmid pUCH1-1
• Accession: NZ_CP096212
• Organism: Enterococcus faecium strain UCH1

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: R2PQC0
• Locus tag: M0E25_RS15860
• Protein ID: WP_002314366.1
• Coordinates: 274770..275042 (-)
• Length: 91 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: Q3Y220
• Locus tag: M0E25_RS15865
• Protein ID: WP_002297422.1
• Coordinates: 275042..275305 (-)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M0E25_RS15820 (M0E25_15820)	269877..270326	-	450	WP_010729386.1	TlpA disulfide reductase family protein	-
M0E25_RS15825 (M0E25_15825)	270482..271024	-	543	WP_002298088.1	hypothetical protein	-
M0E25_RS15830 (M0E25_15830)	271299..271942	+	644	Protein_293	transposase	-
M0E25_RS15835 (M0E25_15835)	272018..272698	+	681	WP_002331383.1	IS6-like element IS1216 family transposase	-
M0E25_RS15840 (M0E25_15840)	272757..272945	-	189	Protein_295	hypothetical protein	-
M0E25_RS15845 (M0E25_15845)	272946..273803	-	858	WP_002292680.1	ParA family protein	-
M0E25_RS15850 (M0E25_15850)	274088..274374	-	287	Protein_297	type II toxin-antitoxin system RelE/ParE family toxin	-
M0E25_RS15855 (M0E25_15855)	274364..274693	-	330	WP_002292678.1	AbrB family transcriptional regulator	-
M0E25_RS15860 (M0E25_15860)	274770..275042	-	273	WP_002314366.1	Txe/YoeB family addiction module toxin	Toxin
M0E25_RS15865 (M0E25_15865)	275042..275305	-	264	WP_002297422.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
M0E25_RS15870 (M0E25_15870)	275542..275946	+	405	WP_002287522.1	IS200/IS605-like element ISEfa4 family transposase	-
M0E25_RS15875 (M0E25_15875)	275963..277111	+	1149	WP_002287525.1	IS200/IS605 family element RNA-guided endonuclease TnpB	-
M0E25_RS15880 (M0E25_15880)	277430..278338	+	909	WP_002295743.1	IS982-like element ISEfm1 family transposase	-
M0E25_RS15885 (M0E25_15885)	278341..279516	-	1176	WP_002336205.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..291523	291523
	-	inside	IScluster/Tn	-	-	263208..289271	26063

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (9-87)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (5-69)

Sequences

Toxin

Protein

• Length: 91 a.a.
• Molecular weight: 10780.24 Da
• Isoelectric Point: 9.9724

>T242880 WP_002314366.1 NZ_CP096212:c275042-274770 [Enterococcus faecium]
MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQSFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEI
YSARTHYESQ

Nucleotide

• Length: 273 bp

>T242880 NZ_CP096212:c275042-274770 [Enterococcus faecium]
ATGAATAACTATTCTGTGATGATTAAAAACTCGGCTAAAGCTGATTTAAAGAAAATCAAACAGTCTAATTTGAAACCTCA
ATTTGAGAAGGTTATTCAGACTTTAAAGGAAGATCCCTATCTCCCTACCCAATCATTTGAGAAGTTAAAACCTACACATG
AAGGTAGGTATTCTAGAAGATTGAATAGACAACACCGGGTTGTTTATAAGGTAGATGAAGATGAACACGTGGTTGAAATT
TATTCAGCAAGGACGCATTACGAGTCACAGTAA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9959.22 Da
• Isoelectric Point: 4.5370

>AT242880 WP_002297422.1 NZ_CP096212:c275305-275042 [Enterococcus faecium]
MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIGLDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVD
EIDWDNL

Nucleotide

• Length: 264 bp

>AT242880 NZ_CP096212:c275305-275042 [Enterococcus faecium]
ATGGCAAAAACAGTCTTAAATCCTAGCTCAGCACGGAAAAATTTTTATAAGTTGTTGAAAGATGTAAATGAGAATCATAC
GGAAATTGAAATCATTAGTGAGCGCAGTGAAAATAATGCGGTGCTTATTGGTTTGGATGATTGGAAAGCAATCAAAGAAA
CGCTATTACTTGAGCAAATAGGCACTTTGGGTGTTGTTCGTAAGCGAGAAAAAGATGATAGTGGATTTACTAATGTTGAT
GAAATTGATTGGGATAACTTATAA

Structures

Toxin

• AlphaFold DB: A0A829F704

Antitoxin

• AlphaFold DB: A0A829F006