Detailed information of TA system TA242875

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 6649307..6649902
• Replicon: chromosome
• Accession: NZ_CP096207
• Organism: Pseudomonas aeruginosa TBCF10839

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A241XLJ5
• Locus tag: M0D10_RS31195
• Protein ID: WP_003117425.1
• Coordinates: 6649307..6649585 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: M0D10_RS31200
• Protein ID: WP_003113527.1
• Coordinates: 6649597..6649902 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M0D10_RS31175 (M0D10_31110)	6644733..6645971	+	1239	WP_003162396.1	C69 family dipeptidase	-
M0D10_RS31180 (M0D10_31115)	6646033..6646680	+	648	WP_003095021.1	carbonate dehydratase	-
M0D10_RS31185 (M0D10_31120)	6646750..6648978	-	2229	WP_003113525.1	TonB-dependent receptor	-
M0D10_RS31190 (M0D10_31125)	6649126..6649254	+	129	Protein_6162	integrase	-
M0D10_RS31195 (M0D10_31130)	6649307..6649585	+	279	WP_003117425.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
M0D10_RS31200 (M0D10_31135)	6649597..6649902	+	306	WP_003113527.1	HigA family addiction module antitoxin	Antitoxin
M0D10_RS31205 (M0D10_31140)	6651259..6652131	+	873	WP_023122808.1	DUF4868 domain-containing protein	-
M0D10_RS31210 (M0D10_31145)	6652166..6653125	-	960	WP_078459576.1	DNA-processing protein DprA	-
M0D10_RS31215 (M0D10_31150)	6653129..6654565	-	1437	WP_079393696.1	HAD-IIIA family hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10701.28 Da
• Isoelectric Point: 8.5576

>T242875 WP_003117425.1 NZ_CP096207:6649307-6649585 [Pseudomonas aeruginosa TBCF10839]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAARELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T242875 NZ_OW967975:c606725-606261 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGTTCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT242875 WP_003113527.1 NZ_CP096207:6649597-6649902 [Pseudomonas aeruginosa TBCF10839]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT242875 NZ_OW967975:c607060-606725 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: A0A241XLJ5