Detailed information of TA system TA242871

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 3025111..3025886
• Replicon: chromosome
• Accession: NZ_CP096207
• Organism: Pseudomonas aeruginosa TBCF10839

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V6ADY6
• Locus tag: M0D10_RS13985
• Protein ID: WP_009518525.1
• Coordinates: 3025111..3025569 (-)
• Length: 153 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: V6AEP7
• Locus tag: M0D10_RS13990
• Protein ID: WP_023098543.1
• Coordinates: 3025569..3025886 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M0D10_RS13960 (M0D10_13925)	3020452..3021399	+	948	WP_009518520.1	TIGR03756 family integrating conjugative element protein	-
M0D10_RS13965 (M0D10_13930)	3021409..3022803	+	1395	WP_009518521.1	integrating conjugative element protein	-
M0D10_RS13970 (M0D10_13935)	3022800..3023159	+	360	WP_009518522.1	hypothetical protein	-
M0D10_RS13975 (M0D10_13940)	3023174..3024691	+	1518	WP_009518523.1	conjugal transfer protein TraG N-terminal domain-containing protein	-
M0D10_RS13980 (M0D10_13945)	3024707..3025084	-	378	WP_009518524.1	DUF3742 family protein	-
M0D10_RS13985 (M0D10_13950)	3025111..3025569	-	459	WP_009518525.1	type II toxin-antitoxin system YhaV family toxin	Toxin
M0D10_RS13990 (M0D10_13955)	3025569..3025886	-	318	WP_023098543.1	type II toxin-antitoxin system PrlF family antitoxin	Antitoxin
M0D10_RS13995 (M0D10_13960)	3026186..3028003	+	1818	WP_023098542.1	MobH family relaxase	-
M0D10_RS14000 (M0D10_13965)	3027985..3028131	-	147	WP_023098541.1	MerR family DNA-binding transcriptional regulator	-
M0D10_RS14005 (M0D10_13970)	3028280..3029191	-	912	WP_023098540.1	NAD(P)H-binding protein	-
M0D10_RS14010 (M0D10_13975)	3029328..3030716	+	1389	Protein_2773	efflux RND transporter permease subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2963300..3096333	133033

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (8-144)

Antitoxin

• PrlF antitoxin (8-100)

Sequences

Toxin

Protein

• Length: 153 a.a.
• Molecular weight: 17660.02 Da
• Isoelectric Point: 10.1848

>T242871 WP_009518525.1 NZ_CP096207:c3025569-3025111 [Pseudomonas aeruginosa TBCF10839]
MQRHGWTLLFHDCVIEQLQKLHAAARRAQENDPAGFESNANVKLFRALSQLMLDVVPGDPARDEYRQGNTLGPAHRHWRR
AKIGRRFRLFFRYDSKAKVIVYAWVNDEQTLRSSGSKSDPYVVFEKMLGRGNPPDDWHALIQASKQDWSKLE

Nucleotide

• Length: 459 bp

>T242871 NZ_OW967967:c86727-86422 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11170.72 Da
• Isoelectric Point: 4.7182

>AT242871 WP_023098543.1 NZ_CP096207:c3025886-3025569 [Pseudomonas aeruginosa TBCF10839]
MPAIHEVATLTSKGQITLPKPIRQALGVDAGGKLAFDLRGSEVVVTRVDAEHEDPAIGAFLSLLARDIEAGRNVRGLPED
LARAMLEHAGHGVNLDEEIDGKVEL

Nucleotide

• Length: 318 bp

>AT242871 NZ_OW967967:c86947-86729 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATACAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: V6ADY6

Antitoxin

• AlphaFold DB: V6AEP7