Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3161389..3162093 Replicon chromosome
Accession NZ_CP096201
Organism Kluyvera ascorbata strain SK

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag MY052_RS15135 Protein ID WP_167540417.1
Coordinates 3161797..3162093 (-) Length 99 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag MY052_RS15130 Protein ID WP_123651216.1
Coordinates 3161389..3161730 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MY052_RS15095 (MY052_15095) 3156780..3157007 + 228 WP_035896671.1 YccJ family protein -
MY052_RS15100 (MY052_15100) 3157103..3157786 - 684 WP_035896670.1 amino acid ABC transporter permease -
MY052_RS15105 (MY052_15105) 3157776..3158561 - 786 WP_115074642.1 amino acid ABC transporter permease -
MY052_RS15110 (MY052_15110) 3158561..3159379 - 819 WP_202216042.1 amino acid ABC transporter ATP-binding protein -
MY052_RS15115 (MY052_15115) 3159381..3160208 - 828 WP_227675098.1 ABC transporter substrate-binding protein -
MY052_RS15120 (MY052_15120) 3160638..3161048 + 411 WP_035894792.1 hypothetical protein -
MY052_RS15125 (MY052_15125) 3161054..3161305 + 252 WP_123651217.1 late histone H1 -
MY052_RS15130 (MY052_15130) 3161389..3161730 - 342 WP_123651216.1 HigA family addiction module antitoxin Antitoxin
MY052_RS15135 (MY052_15135) 3161797..3162093 - 297 WP_167540417.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MY052_RS15140 (MY052_15140) 3162310..3162888 + 579 WP_035894787.1 TetR/AcrR family transcriptional regulator -
MY052_RS15145 (MY052_15145) 3162909..3163709 + 801 WP_248538950.1 winged helix-turn-helix domain-containing protein -
MY052_RS15150 (MY052_15150) 3163709..3164227 + 519 WP_035894785.1 FidL-like protein -
MY052_RS15155 (MY052_15155) 3164224..3164652 - 429 WP_248538951.1 FosA family fosfomycin resistance glutathione transferase -
MY052_RS15160 (MY052_15160) 3165131..3165409 + 279 WP_001540796.1 BMC domain-containing protein -
MY052_RS15165 (MY052_15165) 3165426..3165710 + 285 WP_115074640.1 BMC domain-containing protein -
MY052_RS15170 (MY052_15170) 3165725..3166003 + 279 WP_015585230.1 BMC domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3151603..3162102 10499


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(7-96)

Antitoxin

Cro/C1-type helix-turn-helix domain

(23-70)


Sequences

Toxin        


Download         Length: 99 a.a.        Molecular weight: 11444.99 Da        Isoelectric Point: 8.4291

>T242859 WP_167540417.1 NZ_CP096201:c3162093-3161797 [Kluyvera ascorbata]
MLVNIGSFRDDWLADFFKHATPHKKIPSDIHASLARKLDIINAAVTYQDLKSPPGNRYEELEGKLKDYSSIRVNKQYRLI
FQWANGKAHDLYLDPHEY

Download         Length: 297 bp

>T242859 NZ_OW967966:2880208-2880315 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGGATTATTACCGCAGTGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 12732.57 Da        Isoelectric Point: 4.8605

>AT242859 WP_123651216.1 NZ_CP096201:c3161730-3161389 [Kluyvera ascorbata]
MNQATRKPTTVGDILLFEYLEPLDLKINDLAEMLQVHRNTVSALVNNNRKLTIDMAFRLAKAFDTSAAFWINLQAAVDVW
ETENDARLQVELNKIITAEDFLAARAKANEKAA

Download         Length: 342 bp

>AT242859 NZ_OW967966:c2880155-2880094 [Escherichia coli]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References