Detailed information of TA system TA242844

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 51653..52303
• Replicon: chromosome
• Accession: NZ_CP096201
• Organism: Kluyvera ascorbata strain SK

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A378GDK7
• Locus tag: MY052_RS00250
• Protein ID: WP_035895036.1
• Coordinates: 51653..51994 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MY052_RS00255
• Protein ID: WP_248538000.1
• Coordinates: 52004..52303 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MY052_RS00220 (MY052_00220)	47206..48597	+	1392	WP_248537998.1	hexose-6-phosphate:phosphate antiporter	-
MY052_RS00225 (MY052_00225)	48641..49543	-	903	WP_248537999.1	LysR family transcriptional regulator	-
MY052_RS00230 (MY052_00230)	49649..50101	+	453	WP_035895033.1	DUF1198 family protein	-
MY052_RS00235 (MY052_00235)	50216..50728	+	513	WP_248538981.1	SRPBCC domain-containing protein	-
MY052_RS00240 (MY052_00240)	50773..51096	-	324	WP_202216920.1	DNA-binding transcriptional regulator	-
MY052_RS00245 (MY052_00245)	51080..51439	-	360	WP_123650993.1	type II toxin-antitoxin system RelE/ParE family toxin	-
MY052_RS00250 (MY052_00250)	51653..51994	+	342	WP_035895036.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MY052_RS00255 (MY052_00255)	52004..52303	+	300	WP_248538000.1	helix-turn-helix transcriptional regulator	Antitoxin
MY052_RS00260 (MY052_00260)	52387..53571	+	1185	WP_248538001.1	purine ribonucleoside efflux pump NepI	-
MY052_RS00265 (MY052_00265)	53701..54510	-	810	WP_202216922.1	phosphonoacetaldehyde hydrolase	-
MY052_RS00270 (MY052_00270)	54520..55623	-	1104	WP_248538002.1	2-aminoethylphosphonate--pyruvate transaminase	-
MY052_RS00275 (MY052_00275)	55765..56484	+	720	WP_035895042.1	phosphonate utilization transcriptional regulator PhnR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-107)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-84)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12980.74 Da
• Isoelectric Point: 5.1972

>T242844 WP_035895036.1 NZ_CP096201:51653-51994 [Kluyvera ascorbata]
MWSVETTDVFDSWFEEQAEALREDVLAAMVILSEYGPQLSRPFADTVNGSAFSNMKELRIQHQGNPIRAFFAFDPSRCAI
VLCAGDKTGLNERRFYKEMIKLADAEYRKHLNK

Nucleotide

• Length: 342 bp

>T242844 NZ_OW967966:c71617-71240 [Escherichia coli]
ATGAATACATTACCCGACACTCACGTACGGGAGACATCGGGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACGATGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10806.61 Da
• Isoelectric Point: 8.6010

>AT242844 WP_248538000.1 NZ_CP096201:52004-52303 [Kluyvera ascorbata]
MATLKELMAKQSPESQARIAEKVEALRQTVTLSMLREELNLSQAELAAAMGVKQPTVAKMEQAENDPRLSTLKRYVAALG
GEISIDVTLPTGKKIAFHL

Nucleotide

• Length: 300 bp

>AT242844 NZ_OW967966:c72041-71664 [Escherichia coli]
GTGTCAGACACACTGCCCGGGACAACGCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAATCGGTTGCATTACCTTGTCGACCGTGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCATACCACCTGGACCAGGCCTTTCTGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACACTGTATGCGAAAGAGCTGACCTGCGAAGCCGATACCCTTGGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCGGCAGCGCCCGCAATCACCGTATAA

Structures

Toxin

• AlphaFold DB: A0A378GDK7