Detailed information of TA system TA242827

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1150233..1150882
• Replicon: chromosome
• Accession: NZ_CP096187
• Organism: Enterobacter roggenkampii isolate C3-17

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A1S2AEP5
• Locus tag: M0769_RS05435
• Protein ID: WP_045416590.1
• Coordinates: 1150520..1150882 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: M0769_RS05430
• Protein ID: WP_123922794.1
• Coordinates: 1150233..1150532 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M0769_RS05420 (M0769_05420)	1146657..1148630	+	1974	WP_123922795.1	PhoX family phosphatase	-
M0769_RS05425 (M0769_05425)	1148734..1150122	+	1389	WP_023343530.1	phenylalanine transporter	-
M0769_RS05430 (M0769_05430)	1150233..1150532	-	300	WP_123922794.1	helix-turn-helix transcriptional regulator	Antitoxin
M0769_RS05435 (M0769_05435)	1150520..1150882	-	363	WP_045416590.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
M0769_RS05440 (M0769_05440)	1151089..1152324	+	1236	WP_181530674.1	MFS transporter	-
M0769_RS05445 (M0769_05445)	1152340..1153365	+	1026	WP_181530672.1	sugar phosphate isomerase/epimerase	-
M0769_RS05450 (M0769_05450)	1153358..1154500	+	1143	WP_181530671.1	Gfo/Idh/MocA family oxidoreductase	-
M0769_RS05455 (M0769_05455)	1154576..1155547	+	972	WP_123922791.1	LacI family DNA-binding transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-107)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-80)

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13908.76 Da
• Isoelectric Point: 5.1960

>T242827 WP_045416590.1 NZ_CP096187:c1150882-1150520 [Enterobacter roggenkampii]
MWNVETTDRFDDWFFEQTTALKEDVLATMHILAEFGPTLGRPYVDTVKDSAYANMKELRIQHAGNPIRAFFAFDPDRKAI
VLCAGDKTGCNQKRFYNEMIALADVEYSRHLADKEAIWQR

Nucleotide

• Length: 363 bp

>T242827 NZ_OW967802:1045404-1045739 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10888.66 Da
• Isoelectric Point: 9.8627

>AT242827 WP_123922794.1 NZ_CP096187:c1150532-1150233 [Enterobacter roggenkampii]
MATLKELIAQQSAESQERIAAKVEVMRRIVALNQLREELNISQTELAAAMGVKQPTLAKIEQPGNDPRLSTLKRYVSALG
GEMSIDVTLPNGKRIAFQI

Nucleotide

• Length: 300 bp

>AT242827 NZ_OW967802:1045156-1045404 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• AlphaFold DB: A0A1S2AEP5