Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3388188..3388762 Replicon chromosome
Accession NZ_CP096169
Organism Shewanella sp. JNE2

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag MZ182_RS15190 Protein ID WP_126757499.1
Coordinates 3388188..3388466 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag MZ182_RS15195 Protein ID WP_216506103.1
Coordinates 3388466..3388762 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MZ182_RS15160 (MZ182_15160) 3383921..3384307 - 387 WP_032469918.1 TraA family conjugative transfer protein -
MZ182_RS15165 (MZ182_15165) 3384304..3384876 - 573 WP_248065726.1 type IV conjugative transfer system lipoprotein TraV -
MZ182_RS15170 (MZ182_15170) 3384951..3386240 - 1290 WP_053410659.1 TraB/VirB10 family protein -
MZ182_RS15175 (MZ182_15175) 3386243..3387139 - 897 WP_248065829.1 type-F conjugative transfer system secretin TraK -
MZ182_RS15180 (MZ182_15180) 3387123..3387749 - 627 WP_248065725.1 type IV conjugative transfer system protein TraE -
MZ182_RS15185 (MZ182_15185) 3387746..3388027 - 282 WP_000433889.1 type IV conjugative transfer system protein TraL -
MZ182_RS15190 (MZ182_15190) 3388188..3388466 + 279 WP_126757499.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MZ182_RS15195 (MZ182_15195) 3388466..3388762 + 297 WP_216506103.1 HigA family addiction module antitoxin Antitoxin
MZ182_RS15200 (MZ182_15200) 3388768..3388923 + 156 WP_162889697.1 hypothetical protein -
MZ182_RS15205 (MZ182_15205) 3388934..3389569 - 636 WP_248065724.1 DUF4400 domain-containing protein -
MZ182_RS15210 (MZ182_15210) 3389556..3390116 - 561 WP_248065723.1 conjugative transfer protein -
MZ182_RS15215 (MZ182_15215) 3390126..3391946 - 1821 WP_222727763.1 conjugative transfer system coupling protein TraD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - adeG 3219491..3418248 198757


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(3-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(18-63)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10564.95 Da        Isoelectric Point: 9.9488

>T242818 WP_126757499.1 NZ_CP096169:3388188-3388466 [Shewanella sp. JNE2]
MIKSFKHKGLEKFYRTGSTAGIQATHAKRLRLILSNLDQAEIADDMDLPGLRLHELSGSRKGTWSVTVNGNWRITFRFNG
RDAEIVNYEDYH

Download         Length: 279 bp

>T242818 NZ_OW967796:c82643-82356 [Escherichia coli]
ATGACTTATACGGTTAAATTCAGGGATGATGCCCTCAGGGAATGGCTCAGACTGGATAAGGCCATTCAGCAGCAGTTCGC
AAAAAAACTAAAAAAATGCAGTGAAAATCCTCATATAGCCTCTGCAAAACTACGTGGATTAAAAGATTGCTACAAAATAA
AACTTCGCGCATCAGGTTTTCGTCTGGTCTATCAGGTGATTGATGACATGTTAATTATTGCGGTTGTCGCAGTAGGTAAA
CGTGAACGTAGTCATGTTTACAATCTCGCCAGCGAGAGAATGAGATAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 10952.70 Da        Isoelectric Point: 6.9888

>AT242818 WP_216506103.1 NZ_CP096169:3388466-3388762 [Shewanella sp. JNE2]
MTMHNPAHPGEILKELVIEPLELTITDVAEHLNVSRKTLSKVLNGRGAITPEMAVRLELAFAKPSADHWLRLQSAYDLSV
LRNQKATLHVQPYELAFG

Download         Length: 297 bp

>AT242818 NZ_OW967796:c82891-82640 [Escherichia coli]
ATGCCCAGTATCATTTTGTCTGACACCAGTGCAAGCGTCAGTGAACTGAAAAAAAATCCAATGGCGACAGTCAGTGCCGG
AGAGGGTTATCCTGTTGCCATTCTTAACCGGAATCAGCCTGCATTCTACTGTGTTCCAGCTGAGCTCTATGAAAGGATGC
TTGATGCCCTAGATGACCAGGAGCTGGTGAAACTGGTTACTGAGCGCAGCAGTCAGCCTCTGCATGATGTGGACCTGGAC
AGTTATTTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References