Detailed information of TA system TA242818

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3388188..3388762
• Replicon: chromosome
• Accession: NZ_CP096169
• Organism: Shewanella sp. JNE2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: MZ182_RS15190
• Protein ID: WP_126757499.1
• Coordinates: 3388188..3388466 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MZ182_RS15195
• Protein ID: WP_216506103.1
• Coordinates: 3388466..3388762 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MZ182_RS15160 (MZ182_15160)	3383921..3384307	-	387	WP_032469918.1	TraA family conjugative transfer protein	-
MZ182_RS15165 (MZ182_15165)	3384304..3384876	-	573	WP_248065726.1	type IV conjugative transfer system lipoprotein TraV	-
MZ182_RS15170 (MZ182_15170)	3384951..3386240	-	1290	WP_053410659.1	TraB/VirB10 family protein	-
MZ182_RS15175 (MZ182_15175)	3386243..3387139	-	897	WP_248065829.1	type-F conjugative transfer system secretin TraK	-
MZ182_RS15180 (MZ182_15180)	3387123..3387749	-	627	WP_248065725.1	type IV conjugative transfer system protein TraE	-
MZ182_RS15185 (MZ182_15185)	3387746..3388027	-	282	WP_000433889.1	type IV conjugative transfer system protein TraL	-
MZ182_RS15190 (MZ182_15190)	3388188..3388466	+	279	WP_126757499.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MZ182_RS15195 (MZ182_15195)	3388466..3388762	+	297	WP_216506103.1	HigA family addiction module antitoxin	Antitoxin
MZ182_RS15200 (MZ182_15200)	3388768..3388923	+	156	WP_162889697.1	hypothetical protein	-
MZ182_RS15205 (MZ182_15205)	3388934..3389569	-	636	WP_248065724.1	DUF4400 domain-containing protein	-
MZ182_RS15210 (MZ182_15210)	3389556..3390116	-	561	WP_248065723.1	conjugative transfer protein	-
MZ182_RS15215 (MZ182_15215)	3390126..3391946	-	1821	WP_222727763.1	conjugative transfer system coupling protein TraD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	adeG	3219491..3418248	198757

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (3-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-63)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10564.95 Da
• Isoelectric Point: 9.9488

>T242818 WP_126757499.1 NZ_CP096169:3388188-3388466 [Shewanella sp. JNE2]
MIKSFKHKGLEKFYRTGSTAGIQATHAKRLRLILSNLDQAEIADDMDLPGLRLHELSGSRKGTWSVTVNGNWRITFRFNG
RDAEIVNYEDYH

Nucleotide

• Length: 279 bp

>T242818 NZ_OW967796:c82643-82356 [Escherichia coli]
ATGACTTATACGGTTAAATTCAGGGATGATGCCCTCAGGGAATGGCTCAGACTGGATAAGGCCATTCAGCAGCAGTTCGC
AAAAAAACTAAAAAAATGCAGTGAAAATCCTCATATAGCCTCTGCAAAACTACGTGGATTAAAAGATTGCTACAAAATAA
AACTTCGCGCATCAGGTTTTCGTCTGGTCTATCAGGTGATTGATGACATGTTAATTATTGCGGTTGTCGCAGTAGGTAAA
CGTGAACGTAGTCATGTTTACAATCTCGCCAGCGAGAGAATGAGATAG

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 10952.70 Da
• Isoelectric Point: 6.9888

>AT242818 WP_216506103.1 NZ_CP096169:3388466-3388762 [Shewanella sp. JNE2]
MTMHNPAHPGEILKELVIEPLELTITDVAEHLNVSRKTLSKVLNGRGAITPEMAVRLELAFAKPSADHWLRLQSAYDLSV
LRNQKATLHVQPYELAFG

Nucleotide

• Length: 297 bp

>AT242818 NZ_OW967796:c82891-82640 [Escherichia coli]
ATGCCCAGTATCATTTTGTCTGACACCAGTGCAAGCGTCAGTGAACTGAAAAAAAATCCAATGGCGACAGTCAGTGCCGG
AGAGGGTTATCCTGTTGCCATTCTTAACCGGAATCAGCCTGCATTCTACTGTGTTCCAGCTGAGCTCTATGAAAGGATGC
TTGATGCCCTAGATGACCAGGAGCTGGTGAAACTGGTTACTGAGCGCAGCAGTCAGCCTCTGCATGATGTGGACCTGGAC
AGTTATTTATGA