Detailed information of TA system TA242810

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 114683..115340
• Replicon: plasmid pJNE7-NDM
• Accession: NZ_CP096167
• Organism: Shewanella sp. JNE7

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: MZ097_RS21420
• Protein ID: WP_000270043.1
• Coordinates: 114990..115340 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MZ097_RS21415
• Protein ID: WP_000124640.1
• Coordinates: 114683..114985 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MZ097_RS21370 (MZ097_21375)	110308..110736	+	429	WP_000591074.1	hypothetical protein	-
MZ097_RS21375 (MZ097_21380)	110793..111152	+	360	WP_000422768.1	hypothetical protein	-
MZ097_RS21380 (MZ097_21385)	111152..111598	+	447	WP_000919345.1	Fe3+-siderophore ABC transporter permease	-
MZ097_RS21385 (MZ097_21390)	111595..112113	+	519	WP_000210756.1	nitrite reductase	-
MZ097_RS21390 (MZ097_21395)	112113..112343	+	231	WP_000972663.1	hypothetical protein	-
MZ097_RS21395 (MZ097_21400)	112330..113187	+	858	WP_001167032.1	hypothetical protein	-
MZ097_RS21400 (MZ097_21405)	113418..113945	+	528	WP_001236377.1	thermonuclease family protein	-
MZ097_RS21405 (MZ097_21410)	114003..114275	+	273	WP_001043047.1	HU family DNA-binding protein	-
MZ097_RS21410 (MZ097_21415)	114363..114656	+	294	WP_001239997.1	chromosome segregation protein ParM	-
MZ097_RS21415 (MZ097_21420)	114683..114985	-	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
MZ097_RS21420 (MZ097_21425)	114990..115340	-	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MZ097_RS21425 (MZ097_21430)	115503..116051	+	549	WP_001061574.1	transcriptional regulator	-
MZ097_RS21430 (MZ097_21435)	116392..116586	+	195	WP_000343597.1	hypothetical protein	-
MZ097_RS21435 (MZ097_21440)	116597..116968	+	372	WP_000516916.1	hypothetical protein	-
MZ097_RS21440 (MZ097_21445)	116961..117431	+	471	WP_001281821.1	hypothetical protein	-
MZ097_RS21445 (MZ097_21450)	117446..117781	-	336	WP_000683476.1	hypothetical protein	-
MZ097_RS21450 (MZ097_21455)	117878..118366	+	489	WP_001273096.1	DUF1643 domain-containing protein	-
MZ097_RS21455 (MZ097_21460)	118369..118866	+	498	WP_000062185.1	hypothetical protein	-
MZ097_RS21460 (MZ097_21465)	119081..119785	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aac(3)-IId / dfrA17 / aadA5 / qacE / sul1 / blaNDM-1 / ARR-3	-	1..137224	137224

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T242810 WP_000270043.1 NZ_CP096167:c115340-114990 [Shewanella sp. JNE7]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T242810 NZ_OW967795:2585500-2585607 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTTGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT242810 WP_000124640.1 NZ_CP096167:c114985-114683 [Shewanella sp. JNE7]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT242810 NZ_OW967795:c2585450-2585387 [Escherichia coli]
TCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTATACCTCTCAACGTGCGGGGGTTTTCT

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9