Detailed information of TA system TA242779

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 51322..51907
• Replicon: plasmid pJNE5-64k
• Accession: NZ_CP096122
• Organism: Acinetobacter portensis strain JNE5

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: MZO21_RS14720
• Protein ID: WP_248104264.1
• Coordinates: 51587..51907 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MZO21_RS14715
• Protein ID: WP_248104266.1
• Coordinates: 51322..51594 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MZO21_RS14685 (MZO21_14680)	46442..47224	-	783	WP_154773792.1	sulfite exporter TauE/SafE family protein	-
MZO21_RS14690 (MZO21_14685)	47295..48167	-	873	WP_086044975.1	MBL fold metallo-hydrolase	-
MZO21_RS14695 (MZO21_14690)	48170..48586	-	417	WP_154773791.1	YeeE/YedE thiosulfate transporter family protein	-
MZO21_RS14700 (MZO21_14695)	48587..49003	-	417	WP_154773790.1	YeeE/YedE thiosulfate transporter family protein	-
MZO21_RS14705 (MZO21_14700)	49106..49420	+	315	WP_086044972.1	metalloregulator ArsR/SmtB family transcription factor	-
MZO21_RS14710 (MZO21_14705)	49619..50939	+	1321	Protein_56	ISNCY family transposase	-
MZO21_RS14715 (MZO21_14710)	51322..51594	-	273	WP_248104266.1	NadS family protein	Antitoxin
MZO21_RS14720 (MZO21_14715)	51587..51907	-	321	WP_248104264.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MZO21_RS14725 (MZO21_14720)	52030..53354	-	1325	Protein_59	ISNCY family transposase	-
MZO21_RS14730 (MZO21_14725)	53570..54811	-	1242	WP_228273481.1	mechanosensitive ion channel	-
MZO21_RS14735 (MZO21_14730)	55157..56482	+	1326	WP_248129642.1	ISNCY family transposase	-
MZO21_RS14740 (MZO21_14735)	56591..56805	-	215	Protein_62	ISNCY family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..64629	64629
	-	inside	IScluster/Tn	-	-	28460..64046	35586

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-75)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12224.06 Da
• Isoelectric Point: 7.9834

>T242779 WP_248104264.1 NZ_CP096122:c51907-51587 [Acinetobacter portensis]
MLFIETSIFTKQIKDLVTDEEYRQLQQDLLVQPDKGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLIAY
PKSVKDTLTDKETTILRQLVKEQFHG

Nucleotide

• Length: 321 bp

>T242779 NZ_OW967374:c5269478-5269128 [Klebsiella oxytoca]
ATGAAAACTCTTCCTGCAAATCAGTGGGTGGCGAAGCCATGCCCGCCGCCTGTGCTTGTCTGGCAAACGCTACTTACGCG
TCTGCTTGAGCAACACTATGGCCTGACGCTGAACGACACGCCGTTCAGTGATGAAACCGTTATTCAGGAGCATATCAACG
CCGGTACCACGCTGGCTGATGCCGTTAACTTTCTGGTGGAAAAGTACGAACTGGTTCGTATCGATCGCAGAGGGTTTAAC
TGTCAGGAGCAGTCACCTTATCTTCGGGCTGTAGATATTTTACGGGCAAGGCAGGCAACCGGTTTGCTAACTCAGTGCAA
AAAACGCAAATGTAATCAGCCAGATGGTTGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10123.61 Da
• Isoelectric Point: 9.9218

>AT242779 WP_248104266.1 NZ_CP096122:c51594-51322 [Acinetobacter portensis]
MDNNLFDSLVSSIKEAGSIKRKEIQASRVIELELPNIKEVREKTGLSQVEFAARLHISARTLQNWEQGRRYPTGPAATLI
RILDAHPSVI

Nucleotide

• Length: 273 bp

>AT242779 NZ_OW967374:c5269820-5269503 [Klebsiella oxytoca]
ATGAGCGAACCCTGGTGGGGACTCAGGCGCAGTGTTTCCCCGTGTTTTGGTGCCCGCCTGGTCCAGGAGGGAAACCGCCT
GCATTACCTGGCTGACCGTGCGAACTTTGATGGTCAGTTCTGCGATGCGGATTTGCGCCATCTTGACCAGGCTTTCCCGG
TATTGATGAAACAGCTGGAGTTAATGCTCACCAGCGGTGAACTGAATCCACGGCACCTGCATTGTGTCACGTTGTATGCC
AGAGGGCTGACCTGCGAGGCCGATACGCTGGGTTCGCACGGCTACGTTTATATCGCTATCTATCCCACTCAACGTTAA