Detailed information of TA system TA242772

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 75405..75990
• Replicon: plasmid pJNE5-X3_NDM-1
• Accession: NZ_CP096121
• Organism: Acinetobacter portensis strain JNE5

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N9CJF7
• Locus tag: MZO21_RS13190
• Protein ID: WP_000897307.1
• Coordinates: 75670..75990 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MZO21_RS13185
• Protein ID: WP_000369781.1
• Coordinates: 75405..75677 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MZO21_RS13165 (MZO21_13160)	70917..72392	-	1476	WP_000052512.1	ABC-F type ribosomal protection protein Msr(E)	-
MZO21_RS13170 (MZO21_13165)	72865..73248	+	384	WP_019836977.1	transposase	-
MZO21_RS13175 (MZO21_13170)	73245..73580	+	336	WP_004780063.1	IS66 family insertion sequence element accessory protein TnpB	-
MZO21_RS13180 (MZO21_13175)	73655..75259	+	1605	WP_248129700.1	IS66 family transposase	-
MZO21_RS13185 (MZO21_13180)	75405..75677	-	273	WP_000369781.1	NadS family protein	Antitoxin
MZO21_RS13190 (MZO21_13185)	75670..75990	-	321	WP_000897307.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MZO21_RS13195 (MZO21_13190)	76114..77439	-	1326	WP_139850444.1	ISNCY family transposase	-
MZO21_RS13200 (MZO21_13195)	77617..78036	+	420	WP_248104014.1	SMI1/KNR4 family protein	-
MZO21_RS13205 (MZO21_13200)	78116..78397	-	282	WP_000985609.1	putative addiction module antidote protein	-
MZO21_RS13210 (MZO21_13205)	78398..78700	-	303	WP_000286964.1	type II toxin-antitoxin system RelE/ParE family toxin	-
MZO21_RS13220 (MZO21_13215)	80145..80873	+	729	WP_032073130.1	efflux system response regulator transcription factor AdeR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaCARB-5 / aac(3)-IId / mph(E) / msr(E) / aph(3'')-Ib / tet(X3) / blaNDM-1 / sul2 / lnu(G) / aph(3')-VIa	-	1..333311	333311
	-	inside	IScluster/Tn	aac(3)-IId / mph(E) / msr(E)	-	42971..79872	36901

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-85)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12187.95 Da
• Isoelectric Point: 7.1639

>T242772 WP_000897307.1 NZ_CP096121:c75990-75670 [Acinetobacter portensis]
MLFIETSIFTKQIKDLVSDEEYRQLQQDLLVQPDRGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAY
PKSVKDNLTDKETAILHQLVKEQFHG

Nucleotide

• Length: 321 bp

>T242772 NZ_OW967265:c97919-97389 [Citrobacter freundii]
ATGGAGGGTTTAAGGATTGAGATTTTTTCAGAGGAAGTTGAATATGAATTAAGCAACTTCGATTGTGGAGAAGAGTATCT
GAATACTTTTCTTACTGACCATCTGAAACGGCAGCATAACAGTAAGATCTTACGTGGTTATGTTCTCGTGACGAGAGAGA
ATAAACCGCGTGTGATGGGGTACTACACGCTATCAGGAAGTTGCTTTGAGAAAATACTTCTACCCAGCAAAACACAACAA
AAGCGTGTACCCTACAAGAATGTGCCGAGCGTGACTTTGGGCCGTCTGGCAATTGATAAAAGCATTCACCACCAGGGATA
TGGAGAAACACTTGTTACCCATGCGATGAAAGTCGTGTATCAGGCCTCGCAGGCTGTGGGTATACACGGAATGTTCGTGG
AAGCGCTGAACGACAACGCCAAAAAATTCTACCTTCGACTGGGTTTCATTCAGTTAAAGGAAGAAAACTGTAACTCGCTC
TTTTACCCGACAAAATCTATTGAAGAATTGTTCGAAGTAAATGATGAATAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10149.61 Da
• Isoelectric Point: 8.6582

>AT242772 WP_000369781.1 NZ_CP096121:c75677-75405 [Acinetobacter portensis]
MDNNLFDDLVASIKEAGAIKRKEVKASRVTELELPDIKEVREKTGLSQNEFAARLHISPRTLQNWEQGRRYPTGPAATLI
RILDAHPSLI

Nucleotide

• Length: 273 bp

>AT242772 NZ_OW967265:c98192-97923 [Citrobacter freundii]
ATGTCAGCACTGAAAAAGCAGCGCATTGACCTGAGATTAAATGAAGACGACAAACACATGATCGAAGAAGCTGCAGCAAT
GACTAACCAGTCAATTTCGCAGTTCATGGTCAGTACCGCCTCAGAACGCGCTGCAGAAGTTATTGACCAGCACCGTCGTC
TGCTCCTCAATGAGGAGTCTTGGAACCTGGTTATGGATGCAATCATTAATCCGCCAGCACCGAATGACCGGCTGAAACGC
GCCGCTAACCGTCTCCGGGAACTGGAGTAA

Structures

Toxin

• AlphaFold DB: A0A0A8TMZ3