Detailed information of TA system TA242704

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2229826..2230421
• Replicon: chromosome
• Accession: NZ_CP096017
• Organism: Alkalimarinus coralli strain SCSIO 12638

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: MY524_RS10030
• Protein ID: WP_250655738.1
• Coordinates: 2229826..2230116 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MY524_RS10035
• Protein ID: WP_250655739.1
• Coordinates: 2230131..2230421 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MY524_RS10020	2225328..2227598	-	2271	WP_250655737.1	phosphoenolpyruvate--protein phosphotransferase	-
MY524_RS10025	2227772..2228950	+	1179	WP_250658837.1	tryptophan synthase subunit beta	-
MY524_RS10030	2229826..2230116	+	291	WP_250655738.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MY524_RS10035	2230131..2230421	+	291	WP_250655739.1	HigA family addiction module antitoxin	Antitoxin
MY524_RS10040	2230668..2231984	-	1317	WP_250655740.1	sphingomyelin phosphodiesterase	-
MY524_RS10045	2232290..2233354	-	1065	WP_250655741.1	transposase	-
MY524_RS10050	2233376..2233792	+	417	WP_250655742.1	IS200/IS605 family transposase	-
MY524_RS10055	2233878..2234558	-	681	WP_250655743.1	energy-coupling factor ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (23-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-69)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11028.51 Da
• Isoelectric Point: 7.4018

>T242704 WP_250655738.1 NZ_CP096017:2229826-2230116 [Alkalimarinus coralli]
MIISFGNKATSDLFNGVSSRWVRKLPNQIHELALYKLDVINAAQNLDDLKSPPGNRLELLKGDLEGFHSIRINSQWRIVF
RWDSNAAHDVQIVDYH

Nucleotide

• Length: 291 bp

>T242704 NZ_OW849471:188966-189382 [Klebsiella oxytoca]
ATGACCAAAACCTACATGCTGGATACTAATATCTGCTCTTATATCATGCGCGAACAACCGGAAGCGGTGCTGAAACGCCT
AGAGCAAGCGGTGTTGCGCCGTCACCGGATAGTGGTGTCAGCCATCACCTATGCCGAAATGCGTTTTGGTGCCATCGGTA
AAAGGGCTTCGCCCCGTCATGTGCAGCTGGTGGACGCATTCTGTGCGCGCCTTGATGCCATACTGGCTTGGGATCGGGCC
GCAGTTGACGCTACAACAGAGGTTAAGGCCACGCTAGCAGCTGCCGGCACGCCAATCGGCCCAAACGACACAGCCATTGC
TGGACATGCCATTGCCGCTGGCGCCATCCTGGTGACGAATAATACGCGTGAATTTGAGCGAGTCGCTGGTCTGACGTTTG
AAGACTGGGTGCAATAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10646.34 Da
• Isoelectric Point: 6.8790

>AT242704 WP_250655739.1 NZ_CP096017:2230131-2230421 [Alkalimarinus coralli]
MIPSNRIATHPGVILLKEFLEPLEVTQKALALHLGVPVQRINEIVRGKRGVTPETAWLLSESLNTSPEFWLNLQSIHDLS
ANRPENHIQPLVAAGI

Nucleotide

• Length: 291 bp

>AT242704 NZ_OW849471:188739-188969 [Klebsiella oxytoca]
ATGCGAACTGTATCTGTATTCAGGAACGGTAATAACCGGGCCATCCGTCTGCCCCGCGATCTGGACTTTGACGGCGTGAA
CGAACTGGAAATCATCCGCGAGGGCGATACCCTCATCCTGCGGCCGGTAAAACCCAGTTGGAGTTCGTTCCGCCAGGAGG
AAAAAGCTGACGCAGATTTTATGGCGGAGCGTGAAAATGTTGTTAGTGACGAGGGACGTTTTGAGCTATGA