Detailed information of TA system TA242685

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 5507839..5508425
• Replicon: chromosome
• Accession: NZ_CP095923
• Organism: Pseudomonas aeruginosa strain AR19438

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: MX621_RS25965
• Protein ID: WP_003111576.1
• Coordinates: 5508156..5508425 (-)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A0S3KUN4
• Locus tag: MX621_RS25960
• Protein ID: WP_003111575.1
• Coordinates: 5507839..5508144 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MX621_RS25935 (MX621_25935)	5503993..5504187	+	195	WP_003111573.1	hypothetical protein	-
MX621_RS25940 (MX621_25940)	5504307..5504933	-	627	WP_232772639.1	hypothetical protein	-
MX621_RS25945 (MX621_25945)	5504962..5505990	+	1029	WP_034064933.1	IS30-like element ISPsp7 family transposase	-
MX621_RS25950 (MX621_25950)	5506002..5506856	-	855	WP_232772640.1	hypothetical protein	-
MX621_RS25955 (MX621_25955)	5507119..5507502	-	384	WP_003111574.1	hypothetical protein	-
MX621_RS25960 (MX621_25960)	5507839..5508144	-	306	WP_003111575.1	HigA family addiction module antitoxin	Antitoxin
MX621_RS25965 (MX621_25965)	5508156..5508425	-	270	WP_003111576.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MX621_RS25970 (MX621_25970)	5508478..5508606	-	129	Protein_5145	integrase	-
MX621_RS25975 (MX621_25975)	5508754..5510982	+	2229	WP_023443586.1	TonB-dependent receptor	-
MX621_RS25980 (MX621_25980)	5511053..5511700	-	648	WP_003095021.1	carbonate dehydratase	-
MX621_RS25985 (MX621_25985)	5511762..5513000	-	1239	WP_003111578.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (7-89)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (6-82)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10330.76 Da
• Isoelectric Point: 7.8937

>T242685 WP_003111576.1 NZ_CP095923:c5508425-5508156 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTDAGP
EEVEIVDYH

Nucleotide

• Length: 270 bp

>T242685 NZ_OW849377:2371814-2373136 [Escherichia coli]
ATGCCTAAACTTGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCCAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CAGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCTAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTAACGTTATTACCCGAAAA
TGAAACCATAACGCGCCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGGCTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCTCTGGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCGCAGGAGAAGACAGCGCTG
CTTAGAATAGGCAATGACTGGTGCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGCGAAAT
CAGGCAGCCCAATGCGACGCTCGATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAGGGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATACTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCTGTGAAATA
TGAATCAGATGGAGGCCCAGGCATCGCGCGGATCATGGCGTTTTTGATGGGGTCCAGCGAGGCGCTTCGCGATCGTTATG
ATTTTATGAAATTCCAGGTCTTCCAGTGGTTGATTGGCGCAACGGATGGCCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTTTGGCGACAGCAAAGGTGCTGAGATTCCCGGAAGTGCAGATGCATGAAATCCTAAGTGACTTTGCCAGAATGATTCCG
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAACCAATGTGTT
GAGGTTGCATGGACGGTTAAGCCGAGAATACGGTAGTAAGTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11205.72 Da
• Isoelectric Point: 4.4475

>AT242685 WP_003111575.1 NZ_CP095923:c5508144-5507839 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGEQIEHEIEPLLAQG

Nucleotide

• Length: 306 bp

>AT242685 NZ_OW849377:2371548-2371814 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTAGCGAATGCAATGAAACTGGTTCGCCAGCAAAATGGCTGGAC
GCAGAGCGAGCTGGCGAAAAAAATTGGCATTAAGCAGGCGACAATTTCCAATTTCGAAAACAACCCTGACAATACCACGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACACTATGCGACACGAAAAATGCCTCGCCAGAATCA
ACAGAACAGCAAGATCTGGAGTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0S3KUN4