Detailed information of TA system TA242683

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 4695366..4696047
• Replicon: chromosome
• Accession: NZ_CP095923
• Organism: Pseudomonas aeruginosa strain AR19438

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6AKP0
• Locus tag: MX621_RS22090
• Protein ID: WP_003111825.1
• Coordinates: 4695682..4696047 (-)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MX621_RS22085
• Protein ID: WP_003111824.1
• Coordinates: 4695366..4695689 (-)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MX621_RS22060 (MX621_22060)	4691267..4691905	+	639	WP_003109444.1	hypothetical protein	-
MX621_RS22065 (MX621_22065)	4692148..4692483	+	336	WP_003140884.1	TM2 domain-containing protein	-
MX621_RS22070 (MX621_22070)	4692657..4693175	+	519	WP_020750956.1	PAAR domain-containing protein	-
MX621_RS22075 (MX621_22075)	4693172..4693873	+	702	WP_003111822.1	VRR-NUC domain-containing protein	-
MX621_RS22080 (MX621_22080)	4693890..4694978	+	1089	WP_003111823.1	DUF3396 domain-containing protein	-
MX621_RS22085 (MX621_22085)	4695366..4695689	-	324	WP_003111824.1	XRE family transcriptional regulator	Antitoxin
MX621_RS22090 (MX621_22090)	4695682..4696047	-	366	WP_003111825.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MX621_RS22095 (MX621_22095)	4696340..4696657	-	318	WP_046602310.1	hypothetical protein	-
MX621_RS22100 (MX621_22100)	4696739..4697164	-	426	WP_003111827.1	VOC family protein	-
MX621_RS22105 (MX621_22105)	4697265..4698149	+	885	WP_003111828.1	LysR family transcriptional regulator	-
MX621_RS22110 (MX621_22110)	4698122..4699075	-	954	WP_003085661.1	LysR substrate-binding domain-containing protein	-
MX621_RS22115 (MX621_22115)	4699296..4699730	+	435	WP_003158601.1	RidA family protein	-
MX621_RS22120 (MX621_22120)	4699727..4700974	+	1248	WP_003109738.1	aminotransferase class V-fold PLP-dependent enzyme	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4682738..4698149	15411

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-63)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13880.24 Da
• Isoelectric Point: 4.8219

>T242683 WP_003111825.1 NZ_CP095923:c4696047-4695682 [Pseudomonas aeruginosa]
VAWDIEYTDEFGDWWGSLSEDEQESLAVTVRLLEERGPSLGHPHSSGINGSRHGHMRELRTQHGGRPFRTLYAFDPRRSA
ILLIGGDKTGDDRWYELNVPIADRLYDEHLHQLREEGLIDG

Nucleotide

• Length: 366 bp

>T242683 NZ_OW849377:1836953-1837207 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 11887.72 Da
• Isoelectric Point: 5.8900

>AT242683 WP_003111824.1 NZ_CP095923:c4695689-4695366 [Pseudomonas aeruginosa]
MAKKFSDLRAQMSPEAQARVEAKAQELLAEMPLNELRQARGLSQKMLSEVLQVQQPAIAKMERRTDMYISTLRSHIEAMG
GQLEVIARFPDGAVKISNFADIEEAGA

Nucleotide

• Length: 324 bp

>AT242683 NZ_OW849377:1836705-1836956 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: V6AKP0