Detailed information of TA system TA242677

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5331352..5331947
• Replicon: chromosome
• Accession: NZ_CP095922
• Organism: Pseudomonas aeruginosa strain AR19583

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A241XLJ5
• Locus tag: MX649_RS24660
• Protein ID: WP_003117425.1
• Coordinates: 5331669..5331947 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MX649_RS24655
• Protein ID: WP_003113527.1
• Coordinates: 5331352..5331657 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MX649_RS24625 (MX649_24625)	5326606..5327016	-	411	WP_225877056.1	hypothetical protein	-
MX649_RS24630 (MX649_24630)	5327256..5327543	-	288	WP_088130743.1	DUF5447 family protein	-
MX649_RS24635 (MX649_24635)	5327719..5327991	-	273	WP_004352675.1	hypothetical protein	-
MX649_RS24640 (MX649_24640)	5328101..5328367	+	267	WP_023088595.1	hypothetical protein	-
MX649_RS24645 (MX649_24645)	5328423..5328740	+	318	WP_038405094.1	hypothetical protein	-
MX649_RS24650 (MX649_24650)	5328770..5330968	-	2199	WP_071534174.1	AAA family ATPase	-
MX649_RS24655 (MX649_24655)	5331352..5331657	-	306	WP_003113527.1	HigA family addiction module antitoxin	Antitoxin
MX649_RS24660 (MX649_24660)	5331669..5331947	-	279	WP_003117425.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MX649_RS24665 (MX649_24665)	5332000..5332128	-	129	Protein_4880	integrase	-
MX649_RS24670 (MX649_24670)	5332276..5334504	+	2229	WP_003113525.1	TonB-dependent receptor	-
MX649_RS24675 (MX649_24675)	5334574..5335221	-	648	WP_003095021.1	carbonate dehydratase	-
MX649_RS24680 (MX649_24680)	5335283..5336521	-	1239	WP_014603324.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10701.28 Da
• Isoelectric Point: 8.5576

>T242677 WP_003117425.1 NZ_CP095922:c5331947-5331669 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAARELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T242677 NZ_OW849377:c595011-594547 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT242677 WP_003113527.1 NZ_CP095922:c5331657-5331352 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT242677 NZ_OW849377:c595346-595011 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTCTTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: A0A241XLJ5