Detailed information of TA system TA242671

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5338842..5339437
• Replicon: chromosome
• Accession: NZ_CP095920
• Organism: Pseudomonas aeruginosa strain AR19640

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A241XLJ5
• Locus tag: MX651_RS24755
• Protein ID: WP_003117425.1
• Coordinates: 5339159..5339437 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MX651_RS24750
• Protein ID: WP_003113527.1
• Coordinates: 5338842..5339147 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MX651_RS24715 (MX651_24715)	5334096..5334506	-	411	WP_225877056.1	hypothetical protein	-
MX651_RS24720 (MX651_24720)	5334746..5335033	-	288	WP_088130743.1	DUF5447 family protein	-
MX651_RS24725 (MX651_24725)	5335037..5335219	-	183	WP_047937632.1	hypothetical protein	-
MX651_RS24730 (MX651_24730)	5335209..5335481	-	273	WP_004352675.1	hypothetical protein	-
MX651_RS24735 (MX651_24735)	5335591..5335857	+	267	WP_023088595.1	hypothetical protein	-
MX651_RS24740 (MX651_24740)	5335913..5336230	+	318	WP_038405094.1	hypothetical protein	-
MX651_RS24745 (MX651_24745)	5336260..5338458	-	2199	WP_071534174.1	AAA family ATPase	-
MX651_RS24750 (MX651_24750)	5338842..5339147	-	306	WP_003113527.1	HigA family addiction module antitoxin	Antitoxin
MX651_RS24755 (MX651_24755)	5339159..5339437	-	279	WP_003117425.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MX651_RS24760 (MX651_24760)	5339490..5339618	-	129	Protein_4899	integrase	-
MX651_RS24765 (MX651_24765)	5339766..5341994	+	2229	WP_003113525.1	TonB-dependent receptor	-
MX651_RS24770 (MX651_24770)	5342064..5342711	-	648	WP_003095021.1	carbonate dehydratase	-
MX651_RS24775 (MX651_24775)	5342773..5344011	-	1239	WP_014603324.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10701.28 Da
• Isoelectric Point: 8.5576

>T242671 WP_003117425.1 NZ_CP095920:c5339437-5339159 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAARELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T242671 NZ_OW849195:c4154098-4153757 [Enterobacter cloacae]
ATGAAAACTTTACCTGCAACAACTCTGCGGGTGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGAAAATGTTACTGAC
ACGCCTGCTGGAACAGCACTATGGTCTCACCCTCAACGACACACCGTTCAGTAAGGAACGTGTTATACAGGAACATATCG
ATGCCGGGATCTCGCTGGCTGATGCCGTGAATTTTCTGGTGGAAAAATACGAACTGGTGCGTATCGACAGGAAGGGGTTT
AGCTGGCAGGAACAATCCCCTTATCTCCGGGCTGTAGACATTCTGCGAGCACGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAAAAATTTGGTACGGTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT242671 WP_003113527.1 NZ_CP095920:c5339147-5338842 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT242671 NZ_OW849195:c4154436-4154119 [Enterobacter cloacae]
GTGAATGAAATGACCTGGGGCCTGCAGCGTGACGTCACACCACGCTTCGGTGTCCGGCTGGTGCAGGAGAGCAACCGACT
GCACTATCTGTCTGACCGGGCCAGTATCACCGGTAAGTTCAGTGACGTTGAATGTCTAAGGCTGGATGAAGCATTCCCGC
ACTTTGTCCGTCAGATGGAATTGATGCTGACCACTGGCGATCTGAATCCTCGCCATACACACTGCGTCACCCTGTACCAC
AACGGTTTTACCTGTGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA

Structures

Toxin

• AlphaFold DB: A0A241XLJ5