Detailed information of TA system TA242660

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 49785..50370
• Replicon: plasmid p11J360-1
• Accession: NZ_CP095868
• Organism: Acinetobacter nosocomialis strain 11J360

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N9CJF7
• Locus tag: MW889_RS18900
• Protein ID: WP_000897307.1
• Coordinates: 50050..50370 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MW889_RS18895
• Protein ID: WP_000369781.1
• Coordinates: 49785..50057 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MW889_RS18875 (MW889_18870)	44922..45626	-	705	WP_005028420.1	IS6 family transposase	-
MW889_RS18880 (MW889_18875)	45692..46639	-	948	Protein_52	ISNCY family transposase	-
MW889_RS18885 (MW889_18880)	46877..47761	-	885	WP_000155092.1	Mph(E) family macrolide 2'-phosphotransferase	-
MW889_RS18890 (MW889_18885)	47817..49292	-	1476	WP_000052512.1	ABC-F type ribosomal protection protein Msr(E)	-
MW889_RS18895 (MW889_18890)	49785..50057	-	273	WP_000369781.1	NadS family protein	Antitoxin
MW889_RS18900 (MW889_18895)	50050..50370	-	321	WP_000897307.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MW889_RS18905 (MW889_18900)	50510..50929	+	420	WP_001180106.1	SMI1/KNR4 family protein	-
MW889_RS18910 (MW889_18905)	51009..51290	-	282	WP_000985609.1	putative addiction module antidote protein	-
MW889_RS18915 (MW889_18910)	51291..51593	-	303	WP_000286964.1	type II toxin-antitoxin system RelE/ParE family toxin	-
MW889_RS18920 (MW889_18915)	51742..52125	+	384	WP_257229894.1	hypothetical protein	-
MW889_RS18925 (MW889_18920)	52143..53276	-	1134	WP_171265713.1	IS3-like element ISAba14 family transposase	-
MW889_RS18930 (MW889_18925)	53648..53962	-	315	WP_004728120.1	BrnA antitoxin family protein	-
MW889_RS18935 (MW889_18930)	53949..54236	-	288	WP_000438825.1	BrnT family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	floR / tet(M) / mph(E) / msr(E) / aac(3)-IId / aph(3')-VIa / sul2 / tet(X3)	-	1..285025	285025
	-	inside	IScluster/Tn	floR / tet(M) / mph(E) / msr(E) / aac(3)-IId	-	37568..67377	29809

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-85)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12187.95 Da
• Isoelectric Point: 7.1639

>T242660 WP_000897307.1 NZ_CP095868:c50370-50050 [Acinetobacter nosocomialis]
MLFIETSIFTKQIKDLVSDEEYRQLQQDLLVQPDRGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAY
PKSVKDNLTDKETAILHQLVKEQFHG

Nucleotide

• Length: 321 bp

>T242660 NZ_OW849099:c2890868-2890536 [Citrobacter freundii]
ATGGACAGAGGGGAAATCTGGCTCGTCTCACTGGATCCTACAGCCGGACATGAGCAAAGCGGAAAACGTCCGGTGCTTAT
CGTTTCTCCAGCATCATTTAATAAGTTCACCCGGCTACCTGTTGTCGTTCCCGTCACTAGCGGCGGAAACTTTGCCCGCG
CAGCCGGTTTTACCGTCTCCCTGGACGGTGCGGGAACAAAAACAACGGGTGTTATTCGCTGCGACCAACCCAGAACGATT
GATATGGGGGCTCGGAACGGTAAGTGTCTGGAACGTATACCCGAAGCTGTTGTAAATGAAGTGCTGGCCCGACTGGAAGC
CATTCTAAGCTGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10149.61 Da
• Isoelectric Point: 8.6582

>AT242660 WP_000369781.1 NZ_CP095868:c50057-49785 [Acinetobacter nosocomialis]
MDNNLFDDLVASIKEAGAIKRKEVKASRVTELELPDIKEVREKTGLSQNEFAARLHISPRTLQNWEQGRRYPTGPAATLI
RILDAHPSLI

Nucleotide

• Length: 273 bp

>AT242660 NZ_OW849099:c2891126-2890869 [Citrobacter freundii]
ATGTATACCACTCGCCTGAAAAAGGTCGGCGGATCCATCATGCTGGCGGTTCCTCCTGCCGTGCTGAAAACGCTGGAGCT
GTCGACGGACAGCGAAGTGGGTATGACCATTGATAATGGCTGCCTGATTATTGAACCCCAGAAACGACCTCGTTATTCGC
TTGAGGAACTGCTGGCGCAGTGCGATCCGCACGCCGAAATGAGCGATGAGGATAGGGAATGGATTGATGCGCCTGCAGTG
GGTAAGGAGATCCTGTAA

Structures

Toxin

• AlphaFold DB: A0A0A8TMZ3