Detailed information of TA system TA242648

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4097030..4097724
• Replicon: chromosome
• Accession: NZ_CP095856
• Organism: Escherichia coli strain EC6563

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: MYG04_RS19675
• Protein ID: WP_001263493.1
• Coordinates: 4097326..4097724 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: MYG04_RS19670
• Protein ID: WP_000554757.1
• Coordinates: 4097030..4097323 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MYG04_RS19650 (4092670)	4092670..4093167	+	498	WP_000006260.1	REP-associated tyrosine transposase RayT	-
MYG04_RS19655 (4093383)	4093383..4095095	-	1713	Protein_3844	flagellar biosynthesis protein FlhA	-
MYG04_RS19660 (4095067)	4095067..4095852	+	786	WP_000207564.1	putative lateral flagellar export/assembly protein LafU	-
MYG04_RS19665 (4095923)	4095923..4096978	+	1056	WP_001226164.1	DNA polymerase IV	-
MYG04_RS19670 (4097030)	4097030..4097323	+	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
MYG04_RS19675 (4097326)	4097326..4097724	+	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
MYG04_RS19680 (4097734)	4097734..4098186	+	453	WP_001059847.1	GNAT family N-acetyltransferase	-
MYG04_RS19685 (4098432)	4098432..4098635	+	204	Protein_3850	RtcB family protein	-
MYG04_RS19690 (4098634)	4098634..4099155	+	522	Protein_3851	peptide chain release factor H	-
MYG04_RS19695 (4099212)	4099212..4100669	-	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
MYG04_RS19700 (4100930)	4100930..4101388	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
- (4101984)	4101984..4102064	+	81	NuclAT_13	-	-
- (4101984)	4101984..4102064	+	81	NuclAT_13	-	-
- (4101984)	4101984..4102064	+	81	NuclAT_13	-	-
- (4101984)	4101984..4102064	+	81	NuclAT_13	-	-
MYG04_RS19705 (4101480)	4101480..4102724	+	1245	WP_000189541.1	esterase FrsA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T242648 WP_001263493.1 NZ_CP095856:4097326-4097724 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T242648 NZ_OW849082:c4476072-4475788 [Citrobacter freundii]
ATGACTTATGAACTGGAATTCGACCCGAGAGCCTTAAAAGAGTGGCATAAACTGGGTGATACGGTGAAGGCTCAGTTTAA
GAAGAAGTTGGCCGACGTGTTGTTGAACCCCAGAATTGACTCTGCCCGTTTAAACGGTCTTCCAGATTGCTATAAAATTA
AGCTTAAATCGTCCGGCTATCGTCTGCTGTACCAGGTTCGGGATGAGGTTGTGATCGTGTTTGTTGTCGCGGTCGGCAAG
CGAGAACATTCGGCCGTTTATCTCGATGCAAACAAACGGCTTTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT242648 WP_000554757.1 NZ_CP095856:4097030-4097323 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT242648 NZ_OW849082:c4476304-4476062 [Citrobacter freundii]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCTGTGCGATTACTGTTTCAGTACGTTGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACCGTTAGCGACA
GCGAAGATACACTGATTCAGACAGTCCGGGAGAGGCTGGCCAGCCCGCAAAAAGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6