Detailed information of TA system TA242639

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 1133663..1134165
• Replicon: chromosome
• Accession: NZ_CP095856
• Organism: Escherichia coli strain EC6563

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: MYG04_RS05725
• Protein ID: WP_001560606.1
• Coordinates: 1133663..1133917 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: S1Q063
• Locus tag: MYG04_RS05730
• Protein ID: WP_001259255.1
• Coordinates: 1133914..1134165 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MYG04_RS05695 (1128678)	1128678..1128905	-	228	WP_000985280.1	thiosulfate utilization sulfurtransferase TsuB/YeeD	-
MYG04_RS05700 (1128919)	1128919..1129977	-	1059	WP_000492315.1	thiosulfate utilization transporter TsuA/YeeE	-
MYG04_RS05705 (1130156)	1130156..1131514	-	1359	WP_000019197.1	putrescine/proton symporter PlaP	-
MYG04_RS05710 (1131504)	1131504..1131566	-	63	WP_010723108.1	membrane protein YoeI	-
MYG04_RS05715 (1131781)	1131781..1132710	-	930	WP_024190947.1	LysR substrate-binding domain-containing protein	-
MYG04_RS05720 (1132756)	1132756..1133580	-	825	WP_000754737.1	SDR family oxidoreductase	-
MYG04_RS05725 (1133663)	1133663..1133917	-	255	WP_001560606.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
MYG04_RS05730 (1133914)	1133914..1134165	-	252	WP_001259255.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
MYG04_RS05735 (1134449)	1134449..1134499	+	51	WP_001364200.1	his operon leader peptide	-
MYG04_RS05740 (1134645)	1134645..1135544	+	900	WP_000131782.1	ATP phosphoribosyltransferase	-
MYG04_RS05745 (1135550)	1135550..1136854	+	1305	WP_108429989.1	histidinol dehydrogenase	-
MYG04_RS05750 (1136851)	1136851..1137921	+	1071	WP_000108973.1	histidinol-phosphate transaminase	-
MYG04_RS05755 (1137921)	1137921..1138988	+	1068	WP_000080136.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10153.60 Da
• Isoelectric Point: 8.8560

>T242639 WP_001560606.1 NZ_CP095856:c1133917-1133663 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEVHRLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T242639 NZ_OW849082:265767-266042 [Citrobacter freundii]
ATGAAGGAAAAAGTGTTGTCTTTACGCAAAAAGCAAAAGAACACGCTGGATCAGCTTTTTAAAGCACCGACGCCGCAGGG
AATCAAGTGGTCGGAAATTGAGTCACTGATCAAGGCGTTAGGTGGCGAAATCAAAGAAGGACGAGGCTCGCGATGTAAAT
TTCTTTTAAATAAGAGCATTGCGAGTTTTCATAGACCACATCCTTCTCCGGATACAGATAAAGGCGCGGTCGAAAACGTA
CGTGACTGGTTAACAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9307.58 Da
• Isoelectric Point: 4.7954

>AT242639 WP_001259255.1 NZ_CP095856:c1134165-1133914 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT242639 NZ_OW849082:266039-266398 [Citrobacter freundii]
ATGATCAAACCCAAAACGCCAAACTCAATGGAAATCGCCGGTCAACCTGCGGTGATTAATTATGTCCCTGAGCTTAATGC
GTTTCGCGGTAAGTTCCTGGGATTGTCAGGCTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAGGGGG
AGATCTCCTTACGAGAGTATCTTGATGATTGCTCTGCCGCAGGGATAGAACCTTACGCAAACCCGGAAAAGCTGAAGACG
TTTACTCTGCGCTATCCAGAGTCTTTCGGAGAACGTTTAAGTTTTGCGGCAGCGGAAGAACAGGTATCAGTCAATACATG
GATCATCGAAACGCTCAGCAAAAGTCTCAAACAGGCATAG

Structures

Antitoxin

• PDB: 2A6Q
• AlphaFold DB: A0A7U9LNR0