Detailed information of TA system TA242620

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 4211014..4211693
• Replicon: chromosome
• Accession: NZ_CP095843
• Organism: Escherichia fergusonii strain EF21QZZ116

Toxin (Protein)

• Gene name: higB
• Uniprot ID: B7LJF3
• Locus tag: MYF53_RS20585
• Protein ID: WP_002431667.1
• Coordinates: 4211014..4211316 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MYF53_RS20590
• Protein ID: WP_000806441.1
• Coordinates: 4211352..4211693 (+)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MYF53_RS20560 (4206390)	4206390..4208042	+	1653	WP_000670595.1	bifunctional UDP-sugar hydrolase/5'-nucleotidase	-
MYF53_RS20565 (4208080)	4208080..4208583	-	504	WP_000667001.1	hypothetical protein	-
MYF53_RS20570 (4208580)	4208580..4209236	-	657	WP_181674165.1	hypothetical protein	-
MYF53_RS20575 (4209404)	4209404..4209883	-	480	WP_181203209.1	Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK	-
MYF53_RS20580 (4210087)	4210087..4210881	-	795	WP_000365145.1	TraB/GumN family protein	-
MYF53_RS20585 (4211014)	4211014..4211316	+	303	WP_002431667.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MYF53_RS20590 (4211352)	4211352..4211693	+	342	WP_000806441.1	HigA family addiction module antitoxin	Antitoxin
MYF53_RS20595 (4211807)	4211807..4214311	-	2505	WP_000083966.1	copper-exporting P-type ATPase CopA	-
MYF53_RS20600 (4214618)	4214618..4215907	+	1290	WP_000102764.1	APC family permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (9-98)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-69)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11811.37 Da
• Isoelectric Point: 10.1572

>T242620 WP_002431667.1 NZ_CP095843:4211014-4211316 [Escherichia fergusonii]
MAQKNNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELYLDPHNY

Nucleotide

• Length: 303 bp

>T242620 NZ_OW849064:2614736-2615200 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 13143.01 Da
• Isoelectric Point: 5.2345

>AT242620 WP_000806441.1 NZ_CP095843:4211352-4211693 [Escherichia fergusonii]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVADYLARREERAKNVA

Nucleotide

• Length: 342 bp

>AT242620 NZ_OW849064:2614401-2614736 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTAACGCTGTACTGACCACTGAATCAAAGGTCACTATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCTGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAT
CCGCAAAAAACTCGTCCATTCGACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGACGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: A0A7K4HYG8