Detailed information of TA system TA242525

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 774121..774716
• Replicon: chromosome
• Accession: NZ_CP095774
• Organism: Pseudomonas aeruginosa strain 34Pae8

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: MX625_RS03630
• Protein ID: WP_003113526.1
• Coordinates: 774121..774399 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MX625_RS03635
• Protein ID: WP_003133769.1
• Coordinates: 774411..774716 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MX625_RS03610	769546..770784	+	1239	WP_003095023.1	C69 family dipeptidase	-
MX625_RS03615	770846..771493	+	648	WP_003095021.1	carbonate dehydratase	-
MX625_RS03620	771564..773792	-	2229	WP_023104079.1	TonB-dependent receptor	-
MX625_RS03625	773940..774068	+	129	Protein_717	integrase	-
MX625_RS03630	774121..774399	+	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MX625_RS03635	774411..774716	+	306	WP_003133769.1	HigA family addiction module antitoxin	Antitoxin
MX625_RS03645	775111..776211	-	1101	WP_003095017.1	redox-regulated ATPase YchF	-
MX625_RS03650	776252..776836	-	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
MX625_RS03655	776878..777492	-	615	WP_003095013.1	50S ribosomal protein L25/general stress protein Ctc	-
MX625_RS03660	777609..778550	-	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
MX625_RS03670	778717..779565	-	849	WP_003095007.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (6-82)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T242525 WP_003113526.1 NZ_CP095774:774121-774399 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T242525 NZ_OW706648:c2988692-2988291 [Salmonella enterica subsp. enterica serovar Typhi]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCATGGCGCGCTCAGTGGATCTCATTGCTCCTTCATGGGCTGGTTGCCGC
GGTGATTTTATTGATGCCCTGGCCGCTGAGTTATACCCCGTTATGGATGATACTACTGTCGCTGGTCGTGTTTGACTGTG
TACGTAGCCAGCGCAGGATTAATACCTGCCAGGGAGAGATCAAGCTACTCATGGACGGGCGCTTACGCTGGCAGGGACAG
GACTGGACGCTCTTGCGTCCTCCCTGGTTACTGAAGAGCGGGATGGTGTTGCGTTTACGCGCAGAGTCTGGACGTCATCA
GCATTTATGGCTGGCGGCGGATAGCATGGAAGAAGCGGAATGGCGTGAGTTGCGTCGGATACTGTTACAGCAGCCGATAT
AG

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11214.73 Da
• Isoelectric Point: 4.6081

>AT242525 WP_003133769.1 NZ_CP095774:774411-774716 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGEQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT242525 NZ_OW706648:c2988939-2988673 [Salmonella enterica subsp. enterica serovar Typhi]
ATGGATATTCACAATAAAGCACGTATTCACTGGGCATGTCGTCGCGGTATGCGCGAACTTGATATTTCCATCATGCCGTT
TTTTGAACATGAGTACGATAGTTTAAGTGATGAAGAAAAGCGTATTTTTGTCCGTTTGTTGCAGAGTGATGACCCGGATT
TATTTAACTGGTTAATGAATCACGGTAAACCGGCTGACGCTGAACTGGAACAGATGGTCCGACTCATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: V6ALY3