Detailed information of TA system TA242523

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 6300697..6301292
• Replicon: chromosome
• Accession: NZ_CP095770
• Organism: Pseudomonas aeruginosa strain 34Pae36

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A241XLJ5
• Locus tag: MX654_RS30405
• Protein ID: WP_003117425.1
• Coordinates: 6301014..6301292 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MX654_RS30400
• Protein ID: WP_003099268.1
• Coordinates: 6300697..6301002 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MX654_RS30365	6295920..6296171	-	252	WP_003115979.1	hypothetical protein	-
MX654_RS30370	6296293..6296727	-	435	WP_023087663.1	hypothetical protein	-
MX654_RS30375	6297243..6297533	-	291	WP_033896069.1	DUF5447 family protein	-
MX654_RS30380	6297709..6297981	-	273	WP_004352675.1	hypothetical protein	-
MX654_RS30385	6298428..6298763	+	336	WP_025297674.1	hypothetical protein	-
MX654_RS30390	6298816..6299907	+	1092	WP_124135598.1	hypothetical protein	-
MX654_RS30395	6299968..6300360	-	393	WP_025297673.1	hypothetical protein	-
MX654_RS30400	6300697..6301002	-	306	WP_003099268.1	HigA family addiction module antitoxin	Antitoxin
MX654_RS30405	6301014..6301292	-	279	WP_003117425.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MX654_RS30410	6301617..6303845	+	2229	WP_003113525.1	TonB-dependent receptor	-
MX654_RS30415	6303915..6304562	-	648	WP_003095021.1	carbonate dehydratase	-
MX654_RS30420	6304624..6305862	-	1239	WP_014603324.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-97)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10701.28 Da
• Isoelectric Point: 8.5576

>T242523 WP_003117425.1 NZ_CP095770:c6301292-6301014 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAARELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T242523 NZ_OW706648:2818101-2818628 [Salmonella enterica subsp. enterica serovar Typhi]
ATGATGTTTACAGACTGGCATGAGGCCGCGATAGGGAAAACCCACAATCGAATGAATTTTGATTGTGGGGATGCCGATCT
GAACCAATTCCTGCAACGCCATGCACGACAAAATCATGAGAAAGGGACAACAAAAACCTACGTTGCGCTTGATAATTCGG
ATGTTACACGTATCCACGGCTTTTACTCAGTCAGTCCTGCATCACTGATATATGCACAGGTTCCCGGCGCAATCAGCAAA
GGATTAGGGAGATATGATGTGCCAGTGTTTCGTCTGGGTCGTTTAGCCGTAGACAAATCTATGCAGGGGCAAGGGCTGGG
AGCACAACTTTTGTTATCTGCCGGAAAGCGTTGCATACAGGCGGCTTTGCAGGTCGGTGGCGTAGCCCTGCTTATTGATG
CCAAAAATAAACAGGTCTGCGACTGGTACAAAGGATTTGGCGCAGTACCATTAAACGATCAACCCCTTTCCTTGTTGCTG
TCGTTTAAAACGCTTTATGCTGCTTTATCTGCATCTGGTAGGTTATGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11213.79 Da
• Isoelectric Point: 5.0747

>AT242523 WP_003099268.1 NZ_CP095770:c6301002-6300697 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT242523 NZ_OW706648:2817814-2818104 [Salmonella enterica subsp. enterica serovar Typhi]
ATGAAAACCATGCCTCAGATAGCTATAGAATCTAACGAAAGATTGTCGTTACGTGTATCGACGGATGCCAAAAAGTTGAT
AGTCCGCGCTGCGGCAATCCAGCAAACCAACCTGACGGATTTTGTGGTCAGTAACGTACTACCTGTCGCACAGAAGATTG
TTGATGCCGCCGAACGGGTATATCTGACGGAGCGTGATACTCAAATGATCATGGAAATTTTAGATAACCCGCCAGCGCCG
AATGAAAAGCTTCTTGCCGCTGCTTTCGCCCTGCCGGACATGAAGAAATGA

Structures

Toxin

• AlphaFold DB: A0A241XLJ5