Detailed information of TA system TA242497

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 10384..11041
• Replicon: plasmid pKP5362-1
• Accession: NZ_CP095763
• Organism: Klebsiella pneumoniae strain KP5362

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: MYA94_RS26080
• Protein ID: WP_000270043.1
• Coordinates: 10691..11041 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MYA94_RS26075
• Protein ID: WP_000124640.1
• Coordinates: 10384..10686 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MYA94_RS26030 (MYA94_26030)	6009..6437	+	429	WP_000591074.1	hypothetical protein	-
MYA94_RS26035 (MYA94_26035)	6494..6853	+	360	WP_000422768.1	hypothetical protein	-
MYA94_RS26040 (MYA94_26040)	6853..7299	+	447	WP_000919345.1	Fe3+-siderophore ABC transporter permease	-
MYA94_RS26045 (MYA94_26045)	7296..7814	+	519	WP_000210756.1	nitrite reductase	-
MYA94_RS26050 (MYA94_26050)	7814..8044	+	231	WP_000972663.1	hypothetical protein	-
MYA94_RS26055 (MYA94_26055)	8031..8888	+	858	WP_001167032.1	hypothetical protein	-
MYA94_RS26060 (MYA94_26060)	9119..9646	+	528	WP_001236377.1	thermonuclease family protein	-
MYA94_RS26065 (MYA94_26065)	9704..9976	+	273	WP_001043047.1	HU family DNA-binding protein	-
MYA94_RS26070 (MYA94_26070)	10064..10357	+	294	WP_001239997.1	chromosome segregation protein ParM	-
MYA94_RS26075 (MYA94_26075)	10384..10686	-	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
MYA94_RS26080 (MYA94_26080)	10691..11041	-	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MYA94_RS26085 (MYA94_26085)	11204..11752	+	549	WP_001061574.1	transcriptional regulator	-
MYA94_RS26090 (MYA94_26090)	12093..12287	+	195	WP_000343597.1	hypothetical protein	-
MYA94_RS26095 (MYA94_26095)	12298..12669	+	372	WP_000516916.1	hypothetical protein	-
MYA94_RS26100 (MYA94_26100)	12662..13132	+	471	WP_001281821.1	hypothetical protein	-
MYA94_RS26105 (MYA94_26105)	13147..13482	-	336	WP_000683476.1	hypothetical protein	-
MYA94_RS26110 (MYA94_26110)	13579..14067	+	489	WP_001273096.1	DUF1643 domain-containing protein	-
MYA94_RS26115 (MYA94_26115)	14070..14567	+	498	WP_000062185.1	hypothetical protein	-
MYA94_RS26120 (MYA94_26120)	14782..15486	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aac(3)-IId / aac(6')-Ib-cr / ARR-3 / dfrA27 / aadA16 / qacE / sul1 / blaNDM-1 / aph(6)-Id / aph(3'')-Ib / sul2	-	1..143840	143840

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T242497 WP_000270043.1 NZ_CP095763:c11041-10691 [Klebsiella pneumoniae]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T242497 NZ_OW704627:190678-191205 [Salmonella enterica subsp. enterica serovar Typhi]
ATGATGTTTACAGACTGGCATGAGGCCGCGATAGGGAAAACCCACAATCGAATGAATTTTGATTGTGGGGATGCCGATCT
GAACCAATTCCTGCAACGCCATGCACGACAAAATCATGAGAAAGGGACAACAAAAACCTACGTTGCGCTTGATAATTCGG
ATGTTACACGTATCCACGGCTTTTACTCAGTCAGTCCTGCATCACTGATATATGCACAGGTTCCCGGCGCAATCAGCAAA
GGATTAGGGAGATATGATGTGCCAGTGTTTCGTCTGGGTCGTTTAGCCGTAGACAAATCTATGCAGGGGCAAGGGCTGGG
AGCACAACTTTTGTTATCTGCCGGAAAGCGTTGCATACAGGCGGCTTTGCAGGTCGGTGGCGTAGCCCTGCTTATTGATG
CCAAAAATAAACAGGTCTGCGACTGGTACAAAGGATTTGGCGCAGTACCATTAAACGATCAACCCCTTTCCTTGTTGCTG
TCGTTTAAAACGCTTTATGCTGCTTTATCTGCATCTGGTAGGTTATGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT242497 WP_000124640.1 NZ_CP095763:c10686-10384 [Klebsiella pneumoniae]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT242497 NZ_OW704627:190391-190681 [Salmonella enterica subsp. enterica serovar Typhi]
ATGAAAACCATGCCTCAGATAGCTATAGAATCTAACGAAAGATTGTCGTTACGTGTATCGACGGATGCCAAAAAGTTGAT
AGTCCGCGCTGCGGCAATCCAGCAAACCAACCTGACGGATTTTGTGGTCAGTAACGTACTACCTGTCGCACAGAAGATTG
TTGATGCCGCCGAACGGGTATATCTGACGGAGCGTGATACTCAAATGATCATGGAAATTTTAGATAACCCGCCAGCGCCG
AATGAAAAGCTTCTTGCCGCTGCTTTCGCCCTGCCGGACATGAAGAAATGA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9