Detailed information of TA system TA242490

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 3876899..3877496
• Replicon: chromosome
• Accession: NZ_CP095762
• Organism: Klebsiella pneumoniae strain KP5362

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A9J6S5A1
• Locus tag: MYA94_RS19145
• Protein ID: WP_004893639.1
• Coordinates: 3877179..3877496 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: R4YH91
• Locus tag: MYA94_RS19140
• Protein ID: WP_004142561.1
• Coordinates: 3876899..3877186 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MYA94_RS19110 (3873106)	3873106..3873354	+	249	WP_002893037.1	DUF1158 domain-containing protein	-
MYA94_RS19115 (3873372)	3873372..3873713	-	342	WP_002893035.1	RamA family antibiotic efflux transcriptional regulator	-
MYA94_RS19120 (3873744)	3873744..3874859	-	1116	WP_074180844.1	MBL fold metallo-hydrolase	-
MYA94_RS19125 (3875039)	3875039..3875620	+	582	WP_004176968.1	TetR/AcrR family transcriptional regulator	-
MYA94_RS19130 (3875620)	3875620..3875988	+	369	WP_004142557.1	MmcQ/YjbR family DNA-binding protein	-
MYA94_RS19135 (3876108)	3876108..3876761	+	654	WP_004178896.1	oxygen-insensitive NAD(P)H nitroreductase	-
MYA94_RS19140 (3876899)	3876899..3877186	-	288	WP_004142561.1	helix-turn-helix transcriptional regulator	Antitoxin
MYA94_RS19145 (3877179)	3877179..3877496	-	318	WP_004893639.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MYA94_RS19150 (3877681)	3877681..3878724	-	1044	WP_023279204.1	DUF2157 domain-containing protein	-
MYA94_RS19155 (3879394)	3879394..3880260	-	867	WP_004151823.1	helix-turn-helix transcriptional regulator	-
MYA94_RS19160 (3880369)	3880369..3881796	+	1428	WP_004176980.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-88)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12100.35 Da
• Isoelectric Point: 11.2767

>T242490 WP_004893639.1 NZ_CP095762:c3877496-3877179 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPSIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T242490 NZ_OW704291:c3447267-3446980 [Salmonella enterica subsp. enterica serovar Typhi]
ATGCCGATGGAGTTTGAATGGGATGCGAACAAGGCTAAAAGCAACCTGAGGAAGCACGGTGTCCGCTTTGAGGATGCGGT
ACTGGTGTTTGACGATCCCCGACACCTGTCACGTCAGGAGCGTTATGAGAATGGAGAATATCGTTGGCAGACCCTCGGCC
TTGTTCATGGCATTGTGGTCATTCTGGTTGCTCATAGCGTTCGGTTCGAAAGTGGTTTTGAAGTTATCCGTATCATCAGC
GCTCGTAAGGCAGACAGAAAAGAGAGGAATCGTTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10771.46 Da
• Isoelectric Point: 10.2212

>AT242490 WP_004142561.1 NZ_CP095762:c3877186-3876899 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Nucleotide

• Length: 288 bp

>AT242490 NZ_OW704291:c3446993-3446691 [Salmonella enterica subsp. enterica serovar Typhi]
ATGAGCATGGTTAAACATAAACGCGGCAACGCATCCGCGCTGAGTGCTCAGCATGAGGCTGAACTGAAGGCGCTGGCGAA
AAAATCAGATGATGAGATTGATTACAGCGACATTCCCGCTTCGGAGGATGGACAGTGGTCTGAAGCAGTGCGTGGTAAGT
TTTTTCGCCCGTTAAAAACTCAGGCCTCGGTGCGTATTGACGCCGACGTTATGGAATGGCTGAAACGGCCAGGAAAAGGC
TACCAGACGCGGCTGAATGCCATTCTGCGCGAAGCGATGCTGCGCGAGCAAAATAAGAAATAG

Structures

Antitoxin

• AlphaFold DB: A0A0S3DIQ1