Detailed information of TA system TA242401

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 4507283..4507962
• Replicon: chromosome
• Accession: NZ_CP095540
• Organism: Escherichia coli strain GN03204

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1PK60
• Locus tag: MW399_RS21840
• Protein ID: WP_000057523.1
• Coordinates: 4507660..4507962 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: S1QAY3
• Locus tag: MW399_RS21835
• Protein ID: WP_000806442.1
• Coordinates: 4507283..4507624 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MW399_RS21825 (4503527)	4503527..4504459	-	933	WP_000883041.1	glutaminase A	-
MW399_RS21830 (4504721)	4504721..4507225	+	2505	WP_000083947.1	copper-exporting P-type ATPase CopA	-
MW399_RS21835 (4507283)	4507283..4507624	-	342	WP_000806442.1	HigA family addiction module antitoxin	Antitoxin
MW399_RS21840 (4507660)	4507660..4507962	-	303	WP_000057523.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MW399_RS21845 (4508095)	4508095..4508889	+	795	WP_000365147.1	TraB/GumN family protein	-
MW399_RS21850 (4509093)	4509093..4509572	+	480	WP_000186633.1	Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK	-
MW399_RS21855 (4509596)	4509596..4510396	+	801	WP_000439798.1	hypothetical protein	-
MW399_RS21860 (4510393)	4510393..4510896	+	504	WP_000667000.1	hypothetical protein	-
MW399_RS21865 (4510934)	4510934..4512586	-	1653	WP_000771748.1	bifunctional UDP-sugar hydrolase/5'-nucleotidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (9-98)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-100)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11809.44 Da
• Isoelectric Point: 10.3980

>T242401 WP_000057523.1 NZ_CP095540:c4507962-4507660 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Nucleotide

• Length: 303 bp

>T242401 NZ_OW052571:c2818711-2817890 [Mycobacterium tuberculosis]
GTGGTGGTAGCCCTCGTAGGCTCAGCAATCGTGGACTTGCATTCACGACCACCGTGGTCGAACAACGCGGTGCGTCGTCT
TGGCGTGGCACTGCGCGACGGAGTTGACCCGCCGGTCGACTGCCCGTCGTACGCCGAGGTGATGCTGTGGCATGCGGACT
TGGCCGCCGAAGTCCAGGACCGGATCGAGGGCCGGAGTTGGTCTGCGTCGGAGTTATTGGTTACCTCACGTGCGAAGAGC
CAAGACACCCTGCTAGCAAAGCTGCGGCGTCGGCCTTACCTGCAACTGAACACCATCCAAGACATCGCAGGTGTCCGCAT
CGATGCCGACCTCCTGCTGGGCGAGCAGACGAGACTTGCTCGCGAGATCGCCGACCACTTCGGTGCTGACCAGCCCGCTA
TTCATGATCTGCGTGACCACCCGCACGCCGGCTACCGGGCCGTTCATGTCTGGCTTCGGTTACCTGCCGGTCGTGTCGAG
ATACAGATTCGCACCATTTTGCAGAGCCTGTGGGCCAACTTCTACGAGCTTCTCGCTGACGCGTACGGTCGGGGCATCCG
CTATGACGAGCGGCCGGAGCAGCTAGCGGCCGGCGTTGTCCCGGCACAGCTTCAAGAGCTGGTAGGGGTTATGCAAGACG
CTTCAGCGGATCTGGCGATGCATGAAGCCGAGTGGCAACACTGTGCAGAGATCGAATACCCCGGCCAGCGGGCGATGGCG
CTTGGCGAGGCGAGCAAGAACAAGGCGACGGTGCTCGCAACGACCAAGTTTAGGCTGGAAAGGGCCATCAATGAGGCCGA
GTCGGCAGGGGGAGGTGGGTGA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 13171.10 Da
• Isoelectric Point: 5.7790

>AT242401 WP_000806442.1 NZ_CP095540:c4507624-4507283 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Nucleotide

• Length: 342 bp

>AT242401 NZ_OW052571:c2817897-2817661 [Mycobacterium tuberculosis]
GTGGGTGAGGTGGCTGGCTATGTCGTCGAATACAACCGGCGCACCCACGTGCGTCGCATCACCGAGTTCGCCACCCCGCA
AGAAGCGATGGAGCACCGGTTGAAGCTGGAAGCCGAGCGCACCGACAGCAATATCGAGATCGTTGCGCTCGTCAGTAAGT
CGTTGGGAACCCTGAAGCAAACGCATTCGCGGTACTTCACTGGTGAAGAGCTGAACGTCGGAAACGGCGCGCGGTAG

Structures

Toxin

• AlphaFold DB: S1PK60

Antitoxin

• PDB: 2EBY
• AlphaFold DB: S1QAY3