Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 1600463..1601262 Replicon chromosome
Accession NZ_CP095540
Organism Escherichia coli strain GN03204

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag MW399_RS07660 Protein ID WP_000347251.1
Coordinates 1600463..1600927 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1PPV5
Locus tag MW399_RS07665 Protein ID WP_001296435.1
Coordinates 1600927..1601262 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MW399_RS07630 (1595464) 1595464..1595898 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
MW399_RS07635 (1595916) 1595916..1596794 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
MW399_RS07640 (1596784) 1596784..1597563 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
MW399_RS07645 (1597574) 1597574..1598047 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
MW399_RS07650 (1598070) 1598070..1599350 - 1281 WP_000681950.1 tagatose-bisphosphate aldolase subunit KbaZ -
MW399_RS07655 (1599599) 1599599..1600408 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
MW399_RS07660 (1600463) 1600463..1600927 - 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
MW399_RS07665 (1600927) 1600927..1601262 - 336 WP_001296435.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
MW399_RS07670 (1601411) 1601411..1602982 - 1572 WP_001273763.1 galactarate dehydratase -
MW399_RS07675 (1603357) 1603357..1604691 + 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
MW399_RS07680 (1604707) 1604707..1605477 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T242386 WP_000347251.1 NZ_CP095540:c1600927-1600463 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T242386 NZ_OW052571:1964106-1964423 [Mycobacterium tuberculosis]
ATGACGCGCAGGCTGCGCGTCCATAACGGGGTTGAAGACGACCTATTCGAGGCGTTTTCCTACTACGCGGACGCGGCGCC
AGATCAGATCGATCGGCTTTACAACTTGTTTGTCGATGCCGTGACGAAGCGGATTCCGCAGGCTCCGAACGCGTTTGCGC
CGTTATTCAAGCACTATCGACACATCTACCTCCGGCCCTTCAGGTACTACGTTGCCTATCGGACGACCGACGAGGCTATC
GACATACTGGCTGTTCGGCACGGAATGGAGAACCCGAACGCGGTCGAGGCTGAGATCTCTGGCCGCACCTTCGAGTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12321.92 Da        Isoelectric Point: 4.5392

>AT242386 WP_001296435.1 NZ_CP095540:c1601262-1600927 [Escherichia coli]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT242386 NZ_OW052571:1963894-1964109 [Mycobacterium tuberculosis]
GTGGTGGTCAACCGGGCATTGCTGGCGAGCGTCGACGCACTGTCGCGTGATGAGCAGATTGAGCTCGTCGAGCACATCAA
CGGAAACCTAGCCGAGGGCATGCATATCAGCGAGGCCAACCAGGCGCTCATCGAAGCGCGGGCCAATGACACCGACGATG
CTCATTGGTCCACCATTGATGACTTCGACAAGCGGATCCGCGCCCGGCTCGGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB S1PPV5

References