Detailed information of TA system TA242369

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3708388..3709038
• Replicon: chromosome
• Accession: NZ_CP095538
• Organism: Escherichia coli strain GN04592

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: MW396_RS18340
• Protein ID: WP_000263532.1
• Coordinates: 3708697..3709038 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: U9YXQ7
• Locus tag: MW396_RS18335
• Protein ID: WP_000212552.1
• Coordinates: 3708388..3708687 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MW396_RS18310 (3703836)	3703836..3704147	-	312	WP_000415004.1	hypothetical protein	-
MW396_RS18315 (3704152)	3704152..3704544	-	393	WP_000285322.1	flagellar export chaperone FliS	-
MW396_RS18320 (3704567)	3704567..3705883	-	1317	WP_000609678.1	flagellar filament capping protein FliD	-
MW396_RS18325 (3706088)	3706088..3707002	-	915	WP_000949083.1	lateral flagellin LafA	-
MW396_RS18330 (3707488)	3707488..3708330	+	843	WP_000022366.1	winged helix-turn-helix domain-containing protein	-
MW396_RS18335 (3708388)	3708388..3708687	-	300	WP_000212552.1	helix-turn-helix transcriptional regulator	Antitoxin
MW396_RS18340 (3708697)	3708697..3709038	-	342	WP_000263532.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MW396_RS18345 (3709114)	3709114..3710091	-	978	WP_001195938.1	hypothetical protein	-
MW396_RS18350 (3710108)	3710108..3711037	-	930	WP_001266799.1	flagellar hook-associated protein FlgL	-
MW396_RS18355 (3711052)	3711052..3712428	-	1377	WP_000367245.1	flagellar hook-associated protein FlgK	-
MW396_RS18360 (3712604)	3712604..3712903	-	300	WP_000867281.1	rod-binding protein	-
MW396_RS18365 (3712903)	3712903..3714003	-	1101	WP_001181874.1	flagellar basal body P-ring protein FlgI	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-107)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-84)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13031.86 Da
• Isoelectric Point: 6.4677

>T242369 WP_000263532.1 NZ_CP095538:c3709038-3708697 [Escherichia coli]
MWDVETTETFDNWFDAQTVALKEDLLAAMLILAEYGPQLGRPFADTVNDSQFSNMKELRVQHQGNPVRAFFAFDPARRGI
VLCAGDKTGLNEKKFYRDMIKLADSEYRKHLKK

Nucleotide

• Length: 342 bp

>T242369 NZ_OW052571:c593160-592903 [Mycobacterium tuberculosis]
GTGAGAAGCGTCAACTTCGATCCCGATGCCTGGGAGGACTTCTTGTTCTGGCTGGCCGCTGATCGCAAAACGGCCCGTCG
GATCACCCGGTTGATCGGAGAAATTCAGCGTGATCCGTTCAGCGGGATCGGCAAACCCGAGCCGCTCCAAGGTGAGTTGT
CGGGATACTGGTCGCGCCGGATCGACGACGAACACCGGCTGGTGTATCGAGCGGGCGACGACGAAGTCACGATGCTGAAG
GCCCGATACCACTACTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11004.86 Da
• Isoelectric Point: 9.7665

>AT242369 WP_000212552.1 NZ_CP095538:c3708687-3708388 [Escherichia coli]
MATLKELMAKQSPESQVRIAAKVEEMRQTIALHQLREELNISQTEMAAAMGIKQPTLAKMEQADNDPRLSTLKRYVAALG
GELSIDVKLPNGKRIAFQI

Nucleotide

• Length: 300 bp

>AT242369 NZ_OW052571:c593432-593157 [Mycobacterium tuberculosis]
ATGAGCATCAGTGCGAGCGAGGCGAGGCAGCGCCTGTTTCCACTCATCGAACAGGTCAATACCGATCACCAGCCGGTGCG
GATCACCTCCCGGGCCGGCGATGCGGTGCTGATGTCCGCCGACGACTACGACGCGTGGCAGGAAACGGTCTATCTGCTGC
GCTCACCGGAGAACGCCAGGCGGTTGATGGAAGCGGTTGCCCGGGATAAGGCTGGGCACTCGGCTTTCACCAAGTCTGTA
GATGAGCTGCGGGAGATGGCCGGCGGCGAGGAGTGA

Structures

Antitoxin

• AlphaFold DB: U9YXQ7