Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2233455..2234017 Replicon chromosome
Accession NZ_CP095538
Organism Escherichia coli strain GN04592

Toxin (Protein)


Gene name higB Uniprot ID L4J948
Locus tag MW396_RS10745 Protein ID WP_000605676.1
Coordinates 2233455..2233733 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag MW396_RS10750 Protein ID WP_000781370.1
Coordinates 2233733..2234017 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MW396_RS10720 (2229041) 2229041..2229472 - 432 WP_000152305.1 peroxiredoxin OsmC -
MW396_RS10725 (2229817) 2229817..2230032 + 216 WP_000495766.1 biofilm-dependent modulation protein -
MW396_RS10730 (2230134) 2230134..2230271 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
MW396_RS10735 (2230428) 2230428..2232125 + 1698 WP_000433464.1 malate dehydrogenase -
MW396_RS10740 (2232259) 2232259..2233269 + 1011 WP_000642407.1 alcohol dehydrogenase AdhP -
MW396_RS10745 (2233455) 2233455..2233733 + 279 WP_000605676.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MW396_RS10750 (2233733) 2233733..2234017 + 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
MW396_RS10755 (2234245) 2234245..2234898 - 654 WP_000045646.1 formate dehydrogenase-N subunit gamma -
MW396_RS10760 (2234891) 2234891..2235775 - 885 WP_001240565.1 formate dehydrogenase N subunit beta -
MW396_RS10765 (2235788) 2235788..2238835 - 3048 WP_012602782.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10521.10 Da        Isoelectric Point: 7.3562

>T242364 WP_000605676.1 NZ_CP095538:2233455-2233733 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T242364 NZ_OW052570:4295532-4295966 [Mycobacterium tuberculosis]
ATGAAGATCGTCGACGCGAACGTCTTGCTCTACGCCGTGAACACCACAAGTGAGCACCACAAGCCGTCGCTGCGCTGGCT
TGACGGTGCGCTGTCGGGCGCCGACCGCGTCGGGTTCGCCTGGGTGCCGTTGTTGGCGTTCGTGCGATTGGCGACCAAGG
TGGGGTTGTTCCCCCGTCCGCTTCCGCGGGAGGCGGCCATCACCCAGGTCGCGGATTGGCTAGCCGCACCCAGCGCCGTC
TTGGTGAATCCGACCGTCCGGCACGCCGATATCCTGGCGAGAATGCTGACGTACGTGGGAACCGGTGCCAACCTGGTCAA
CGACGCGCATCTGGCCGCGCTTGCCGTCGAGCATCGCGCCAGCATCGTGTCCTACGACAGTGACTTCGGCCGATTCGAAG
GGGTGCGCTGGGATCAGCCGCCCGCGCTGTTGTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT242364 WP_000781370.1 NZ_CP095538:2233733-2234017 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT242364 NZ_OW052570:4295271-4295525 [Mycobacterium tuberculosis]
ATGCGGACGACTGTCACATTGGATGACGACGTCGAGCAGTTGGTAAGGCGCCGGATGGCCGAGCGGCAAGTGTCCTTCAA
GAAGGCCCTCAACGACGCGATTCGCGACGGCGCCTCCGGGCGACCTGCGCCGAGTCACTTCAGCACCCGCACGGCAGACT
TGGGCGTCCCTGCGGTCAACCTCGACCGGGCCTTGCAGCTTGCGGCCGACCTCGAGGACGAAGAGCTTGTGCGCCGTCAA
CGCCGCGGGTCATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G6T1

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References