Detailed information of TA system TA024236

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 828193..828763
• Replicon: chromosome
• Accession: NC_013174
• Organism: Jonesia denitrificans DSM 20603

Toxin (Protein)

• Gene name: graT
• Uniprot ID: C7R1W2
• Locus tag: JDEN_RS03845
• Protein ID: WP_015771058.1
• Coordinates: 828193..828474 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JDEN_RS03850
• Protein ID: WP_015771059.1
• Coordinates: 828461..828763 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JDEN_RS03800	823601..824005	-	405	WP_041288145.1	hypothetical protein	-
JDEN_RS03805	824051..824395	-	345	WP_015771049.1	hypothetical protein	-
JDEN_RS13615	824388..824585	-	198	WP_015771050.1	hypothetical protein	-
JDEN_RS03815	825013..825465	-	453	WP_015771052.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
JDEN_RS03820	825606..825968	-	363	WP_015771053.1	hypothetical protein	-
JDEN_RS03825	826004..826231	-	228	WP_015771054.1	hypothetical protein	-
JDEN_RS03830	826322..826657	-	336	WP_015771055.1	hypothetical protein	-
JDEN_RS03835	826762..827751	-	990	WP_015771056.1	SRPBCC domain-containing protein	-
JDEN_RS03840	827748..827993	-	246	WP_015771057.1	hypothetical protein	-
JDEN_RS03845	828193..828474	+	282	WP_015771058.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JDEN_RS03850	828461..828763	+	303	WP_015771059.1	HigA family addiction module antidote protein	Antitoxin
JDEN_RS03855	828760..829275	+	516	WP_015771060.1	GNAT family N-acetyltransferase	-
JDEN_RS03860	829365..829751	-	387	WP_015771061.1	hypothetical protein	-
JDEN_RS03865	829789..830076	-	288	WP_015771062.1	transcriptional regulator	-
JDEN_RS03870	830078..830398	-	321	WP_143713239.1	hypothetical protein	-
JDEN_RS03875	830505..830759	+	255	WP_015771064.1	type II toxin-antitoxin system RelE/ParE family toxin	-
JDEN_RS03880	830881..831534	+	654	WP_015771065.1	hypothetical protein	-
JDEN_RS03885	832004..832845	+	842	Protein_753	NAD(P)-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	818269..836693	18424

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (24-70)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10927.43 Da
• Isoelectric Point: 10.9360

>T24236 WP_015771058.1 NC_013174:828193-828474 [Jonesia denitrificans DSM 20603]
VIRSFGDKETERLWRRERVRSIDPRIHRVALRKLRQVGSAESIEDLRVPPGNRLEALKGNRSGQHSIRINDQWRICFVWT
AAGPEEVQIVDYH

Nucleotide

• Length: 282 bp

>T24236 NC_013174:828193-828474 [Jonesia denitrificans DSM 20603]
GTGATCAGATCGTTCGGCGACAAGGAGACCGAACGGCTGTGGCGTCGTGAGCGCGTGCGCTCGATCGATCCGAGGATTCA
TCGGGTCGCGCTGCGCAAACTGCGTCAGGTGGGGTCCGCGGAGTCCATCGAGGACCTCCGTGTCCCACCCGGTAACCGCC
TCGAAGCTCTCAAGGGCAACCGATCTGGTCAGCACAGCATCAGGATCAACGACCAGTGGCGGATCTGCTTCGTGTGGACC
GCCGCCGGACCAGAGGAGGTGCAGATCGTTGACTACCACTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11063.60 Da
• Isoelectric Point: 4.8129

>AT24236 WP_015771059.1 NC_013174:828461-828763 [Jonesia denitrificans DSM 20603]
MTTTDKIEPIHPGEVLMEDFIEGFGITQNKLAVSIGVPPRRINEIVHGKRGITADTALRLAKYFGTSAEFWINLQSHYEL
DRAEDLAGEQIASITPLQVA

Nucleotide

• Length: 303 bp

>AT24236 NC_013174:828461-828763 [Jonesia denitrificans DSM 20603]
TTGACTACCACTGACAAGATCGAGCCGATCCACCCGGGCGAGGTGCTCATGGAGGACTTCATCGAGGGCTTCGGCATCAC
GCAGAACAAGCTCGCCGTATCCATCGGCGTTCCGCCGCGACGGATCAACGAGATCGTGCACGGCAAGCGAGGGATCACCG
CAGACACGGCGCTGCGGCTCGCGAAGTACTTCGGCACCTCGGCCGAGTTCTGGATCAACCTGCAGAGCCACTACGAGCTG
GACCGTGCGGAGGACCTCGCGGGCGAGCAGATCGCGTCGATCACGCCACTGCAGGTCGCGTGA

Structures

Toxin

• AlphaFold DB: C7R1W2