Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1618418..1618920 Replicon chromosome
Accession NZ_CP095538
Organism Escherichia coli strain GN04592

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag MW396_RS07650 Protein ID WP_000767829.1
Coordinates 1618666..1618920 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag MW396_RS07645 Protein ID WP_001259255.1
Coordinates 1618418..1618669 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MW396_RS07620 (1613596) 1613596..1614663 - 1068 WP_000080135.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
MW396_RS07625 (1614663) 1614663..1615733 - 1071 WP_000108941.1 histidinol-phosphate transaminase -
MW396_RS07630 (1615736) 1615736..1617034 - 1299 WP_000009595.1 histidinol dehydrogenase -
MW396_RS07635 (1617040) 1617040..1617939 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
MW396_RS07640 (1618085) 1618085..1618135 - 51 WP_001364200.1 his operon leader peptide -
MW396_RS07645 (1618418) 1618418..1618669 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
MW396_RS07650 (1618666) 1618666..1618920 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
MW396_RS07655 (1619003) 1619003..1619827 + 825 WP_000754737.1 SDR family oxidoreductase -
MW396_RS07660 (1619873) 1619873..1620802 + 930 WP_000803351.1 LysR substrate-binding domain-containing protein -
MW396_RS07665 (1621017) 1621017..1621079 + 63 WP_010723108.1 membrane protein YoeI -
MW396_RS07670 (1621069) 1621069..1622427 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
MW396_RS07675 (1622606) 1622606..1623664 + 1059 WP_000492339.1 thiosulfate utilization transporter TsuA/YeeE -
MW396_RS07680 (1623678) 1623678..1623905 + 228 WP_000234895.1 thiosulfate utilization sulfurtransferase TsuB/YeeD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T242353 WP_000767829.1 NZ_CP095538:1618666-1618920 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T242353 NZ_OW052570:3535595-3536479 [Mycobacterium tuberculosis]
GTGGCCGGTCTGACCCGTGCGCTCGTTGCGCGTCACGCTCTTGGTCGGGCCGAGGCATACGACGCCGCTCTGCTCGACGT
CGCCCAGGACCACCTGCTGTACCTGCTGTCGCAGACCGTCCAGTTCGGCGATAACCGCCTGGTGTTCAAAGGCGGTACCA
GCTTACGGAAGTGCCGGCTGGGCAACGTCGGGCGGTTCTCCACTGATCTGGACTTCAGCGCGCCCGACGATGAGGTCGTG
CTGGAGGTGTGCGAGCTGATCGACGGCGCCCGTGTCGGCGGCTTCGAATTCGGCGTGCAGAGCACACGAGGCGACGGCCG
GCATTGGCAGTTGCGGGTGCGGCACACGGAATTGGGCGAGCCCCGGATCGTCGCCAGCGTCGAGTTCGCACGGCGGCCGT
TGGCTTTGCCGTCTGAGCTGCTCGCATTCATCCAGTTGCCGATCCATAAGGCTTACGGTTTTGGGCTGCCGACACTGCCG
GTCGTGGCAGAAGCGGAAGCCTGCGCAGAGAAACTCGCGCGGTACCGCCGCGTTGCGCTGGCACGGGACCTGTACGACCT
GAACCACTTCGCCTCGCGAACGATTGACGAACCGCTCGTGCGGCGGCTGTGGGTGCTCAAGGTGTGGGGTGATGTCGTCG
ATGACCGGCGCGGCACCCGGCCACTACGCGTCGAAGACGTCCTCGCCGCCCGCAGCGAGCACGACTTCCAGCCCGACTCG
ATCGGCGTGCTGACCCGTCCTGTCGCTATGGCTGCCTGGGAAGCTCGCGTTCGGAAGCGATTTGCGTTCCTCACTGACCT
CGACGCCGACGAGCAGCGGTGGGCCGCCTGCGACGAACGGCACCGCCGCGAAGTGGAGAACGCGCTGGCGGTGCTGCGGT
CCTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT242353 WP_001259255.1 NZ_CP095538:1618418-1618669 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT242353 NZ_OW052570:3534705-3535592 [Mycobacterium tuberculosis]
GTGGTGAGCCCAGCCGGCGCCGATCGGCGCATCCCCACGTGGGCGTCACGGGTGGTGTCCGGCCTCGCTCGCGATCGGCC
CGTAGTCGTCACGAAAGAGGATCTGACGCAACGGCTTACAGAGGCTGGGTGTGGACGCGACCCCGACTCCGCCATCCGCG
AACTGCGCCGCATTGGGTGGCTGGTCCAACTGCCGGTGAAAGGCACCTGGGCATTCATTCCCCCCGGCGAGGCCGCCATC
TCGGACCCGTATCTACCGCTGCGCTCGTGGCTGGCCCGTGACCAGAACGCGGGCTTCATGCTGGCCGGTGCATCCGCAGC
GTGGCACCTCGGATACCTGGACCGCCAACCCGACGGCCGCATCCCGATCTGGCTGCCGCCGGCCAAAAGGCTGCCCGACG
GCCTAGCATCGTATGTGTCCGTCGTGCGCATCCCCTGGAACGCGGCGGACACCGCACTGCTGGCTCCGCGTCCCGCGTTG
CTGGTCCGGCGGCGGCTCGACCTCGTCGCGTGGGCGACCGGGTTACCGGCGCTAGGACCAGAAGCATTACTTGTGCAAAT
CGCCACGCGCCCGGCCTCGTTCGGGCCGTGGGCCGACCTTGTCCCCCACCTTGACGACCTCGTCGCCGACTGCAGTGACG
AGCGCCTGGAGCGTCTACTGTCGGGCCGGCCGACATCGGCCTGGCAGCGTGCAAGCTACCTGCTGGACTCGGGCGGTGAA
CCGGCACGAGGCCAGGCGCTGCTGGCCAAGCGGCACACCGAAGTGATGCCGGTGACTCGATTCACCACCGCGCACAGCCG
CGACCGTGGCGAGAGTGTCTGGGCTCCCGAGTATCAGCTTGTCGACGAGCTCGTCGTACCGCTGCTGCGCGTGATCGGCA
AGGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References