Detailed information of TA system TA242350

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 378973..379666
• Replicon: chromosome
• Accession: NZ_CP095538
• Organism: Escherichia coli strain GN04592

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: S1EZG2
• Locus tag: MW396_RS01955
• Protein ID: WP_000415584.1
• Coordinates: 378973..379269 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: S1EBV2
• Locus tag: MW396_RS01960
• Protein ID: WP_000650107.1
• Coordinates: 379271..379666 (+)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MW396_RS01920 (374052)	374052..374366	-	315	WP_000958598.1	putative quinol monooxygenase	-
MW396_RS01925 (374397)	374397..374978	-	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
MW396_RS01930 (375306)	375306..375638	+	333	WP_000917684.1	DUF2645 family protein	-
MW396_RS01935 (375684)	375684..377033	-	1350	WP_000673402.1	quorum sensing histidine kinase QseC	-
MW396_RS01940 (377030)	377030..377689	-	660	WP_001221493.1	quorum sensing response regulator transcription factor QseB	-
MW396_RS01945 (377841)	377841..378233	+	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
MW396_RS01950 (378286)	378286..378768	+	483	WP_000183505.1	GyrI-like domain-containing protein	-
MW396_RS01955 (378973)	378973..379269	+	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	Toxin
MW396_RS01960 (379271)	379271..379666	+	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	Antitoxin
MW396_RS01965 (379799)	379799..381406	+	1608	WP_001295629.1	ABC transporter substrate-binding protein	-
MW396_RS01970 (381544)	381544..383802	+	2259	WP_001281881.1	DNA topoisomerase IV subunit A	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (5-97)

Antitoxin

• Zinc finger, MqsA-type (3-47)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11231.96 Da
• Isoelectric Point: 8.9070

>T242350 WP_000415584.1 NZ_CP095538:378973-379269 [Escherichia coli]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Nucleotide

• Length: 297 bp

>T242350 NZ_OW052570:c3486806-3486363 [Mycobacterium tuberculosis]
ATGCTGTGCGTTGATGTCAACGTGCTCGTTTACGCGCATCGGGCAGACCTACGGGAGCACGCGGACTATCGGGGTTTGCT
TGAGCGGCTGGCCAACGATGACGAGCCGCTGGGTCTACCAGATAGCGTGCTCGCCGGCTTCATCCGGGTGGTTACCAACC
GCCGCGTCTTCACCGAGCCGACGAGCCCACAGGACGCATGGCAGGCAGTCGACGCCCTACTCGCGGCACCCGCAGCCATG
CGACTTCGGCCTGGCGAGCGCCACTGGATGGCCTTTCGGCAGTTAGCGTCCGATGTTGATGCGAACGGCAACGACATTGC
GGACGCGCACCTGGCCGCCTACGCGCTAGAGAACAACGCAACCTGGTTGAGCGCCGACCGCGGCTTTGCCCGTTTCCGTC
GACTGCGCTGGCGTCATCCGTTGGACGGTCAGACCCATCTATAA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14703.10 Da
• Isoelectric Point: 9.2136

>AT242350 WP_000650107.1 NZ_CP095538:379271-379666 [Escherichia coli]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Nucleotide

• Length: 396 bp

>AT242350 NZ_OW052570:c3487059-3486793 [Mycobacterium tuberculosis]
ATGCCATGCGTACGCACGACGATCCGTATCGATGACGAGCTGTACCGCGAGGTGAAAGCAAAGGCCGCTCGTTCCGGGCG
TACCGTGGCCGCGGTTCTTGAAGATGCGGTGCGGCGTGGTCTCAACCCGCCTAAGCCGCAGGCCGCCGGCCGTTATCGAG
TCCAGCCGTCGGGTAAGGGCGGCCTGCGGCCCGGTGTCGATCTATCGTCCAACGCCGCACTTGCCGAAGCGATGAACGAC
GGCGTGTCGGTCGATGCTGTGCGTTGA

Structures

Toxin

• PDB: 3HI2

Antitoxin

• PDB: 3HI2
• PDB: 3GA8
• PDB: 3O9X
• PDB: 2KZ8
• PDB: 3FMY
• PDB: 3GN5
• AlphaFold DB: A0A7U9LU65