Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 272534..273333 Replicon chromosome
Accession NZ_CP095538
Organism Escherichia coli strain GN04592

Toxin (Protein)


Gene name yhaV Uniprot ID B7NDB6
Locus tag MW396_RS01425 Protein ID WP_000347269.1
Coordinates 272534..272998 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID V0YUB5
Locus tag MW396_RS01430 Protein ID WP_001309780.1
Coordinates 272998..273333 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MW396_RS01395 (267535) 267535..267969 - 435 WP_000948818.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
MW396_RS01400 (267987) 267987..268865 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
MW396_RS01405 (268855) 268855..269634 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
MW396_RS01410 (269645) 269645..270118 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
MW396_RS01415 (270141) 270141..271421 - 1281 WP_000681943.1 tagatose-bisphosphate aldolase subunit KbaZ -
MW396_RS01420 (271670) 271670..272479 + 810 WP_000072171.1 aga operon transcriptional regulator AgaR -
MW396_RS01425 (272534) 272534..272998 - 465 WP_000347269.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
MW396_RS01430 (272998) 272998..273333 - 336 WP_001309780.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
MW396_RS01435 (273482) 273482..275053 - 1572 WP_001273738.1 galactarate dehydratase -
MW396_RS01440 (275428) 275428..276762 + 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
MW396_RS01445 (276778) 276778..277548 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17806.22 Da        Isoelectric Point: 9.6924

>T242348 WP_000347269.1 NZ_CP095538:c272998-272534 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTREAEETH

Download         Length: 465 bp

>T242348 NZ_OW052570:2961394-2961822 [Mycobacterium tuberculosis]
ATGCGAGCGCTGTTGGACGTCAACGTGCTACTGGCGCTGTTGGACCGCGATCACGTCGACCACGAGCGCGCCCGAGCGTG
GATCACCGGGCAGATCGAGCGGGGATGGGCTTCCTGTGCGATCACCCAAAACGGGTTCGTCCGCGTCATCAGCCAACCGC
GCTATCCCAGCCCAATCTCGGTAGCCCATGCCATCGACTTGTTGGCTCGTGCGACGCACACGCGCTATCACGAGTTCTGG
TCCTGCACCGTCAGCATTCTCGACTCAAAGGTCATCGATCGTTCCCGCCTGCACAGCCCCAAACAGGTCACCGACGCGTA
CTTGCTGGCCCTCGCGGTCGCTCATGATGGCCGATTTGTCACCTTCGATCAGTCGATTGCGTTGACTGCGGTTCCTGGCG
CAACCAAACAGCACCTGGCAACGCTGTAG

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12345.94 Da        Isoelectric Point: 4.8616

>AT242348 WP_001309780.1 NZ_CP095538:c273333-272998 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNT
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT242348 NZ_OW052570:2961148-2961390 [Mycobacterium tuberculosis]
ATGCGCACCACGTTGTCCATCGACGACGATGTATTGCTGGCCGTGAAGGAACGGGCACGCAGGGAGAAGCGCACCGCGGG
TGAAATCCTGTCGGATCTGGCCCGGCAAGCGTTAACCAACCAGAATCCCCAGCCTGCGGCGAGCCAGGAGGACGCCTTTC
ATGGCTTCGAACCCCTGCCTCATCGCGGTGGAGCGGTATCGAACGCGCTGATCGATCGGCTTCGTGACGAGGAGGCCGTG
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829IY86

Antitoxin

Source ID Structure
AlphaFold DB V0YUB5

References