Detailed information of TA system TA242345

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 4688544..4689343
• Replicon: chromosome
• Accession: NZ_CP095533
• Organism: Escherichia coli strain GN02461

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: MW400_RS21990
• Protein ID: WP_000347273.1
• Coordinates: 4688879..4689343 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: V0YUB5
• Locus tag: MW400_RS21985
• Protein ID: WP_001309780.1
• Coordinates: 4688544..4688879 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MW400_RS21970 (4684329)	4684329..4685099	-	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-
MW400_RS21975 (4685115)	4685115..4686449	-	1335	WP_000599654.1	galactarate/glucarate/glycerate transporter GarP	-
MW400_RS21980 (4686824)	4686824..4688395	+	1572	WP_001273738.1	galactarate dehydratase	-
MW400_RS21985 (4688544)	4688544..4688879	+	336	WP_001309780.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
MW400_RS21990 (4688879)	4688879..4689343	+	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
MW400_RS21995 (4689398)	4689398..4690207	-	810	WP_000072171.1	aga operon transcriptional regulator AgaR	-
MW400_RS22000 (4690456)	4690456..4691736	+	1281	WP_000681959.1	tagatose-bisphosphate aldolase subunit KbaZ	-
MW400_RS22005 (4691759)	4691759..4692232	+	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
MW400_RS22010 (4692243)	4692243..4693022	+	780	WP_000406214.1	PTS N-acetylgalactosamine transporter subunit IIC	-
MW400_RS22015 (4693012)	4693012..4693890	+	879	WP_001295548.1	PTS N-acetylgalactosamine transporter subunit IID	-
MW400_RS22020 (4693908)	4693908..4694342	+	435	WP_000948818.1	PTS galactosamine/N-acetylgalactosamine transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4679781..4689343	9562

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T242345 WP_000347273.1 NZ_CP095533:4688879-4689343 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T242345 NZ_OW052570:c2843787-2843377 [Mycobacterium tuberculosis]
GTGATCTATATGGACACCTCGGCCCTGACTAAGCTGCTCATCTCCGAGCCCGAGACGACCGAACTGCGGACATGGCTGAC
CGCGCAAAGCGGCCAGGGCGAGGACGCGGCGACAAGCACCCTTGGCCGGGTCGAGTTGATGAGAGTCGTTGCCCGATACG
GACAACCAGGCCAAACTGAGCGTGCGCGTTACCTACTCGACGGGCTCGACATCCTCCCGCTCACCGAACCGGTGATCGGT
CTAGCTGAAACGATCGGACCGGCCACCCTACGTTCTCTCGACGCGATTCACCTCGCGGCCGCAGCCCAGATCAAGCGGGA
ACTGACAGCCTTCGTCACCTACGACCACCGATTGTTGAGCGGATGCCGTGAGGTCGGCTTCGTCACCGCCTCACCCGGCG
CAGTCCGGTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12345.94 Da
• Isoelectric Point: 4.8616

>AT242345 WP_001309780.1 NZ_CP095533:4688544-4688879 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNT
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT242345 NZ_OW052570:c2844062-2843784 [Mycobacterium tuberculosis]
GTGGAGGCAATCGGAATCCGAGAACTAAGACAGCACGCATCGCGATACCTCGCCCGGGTTGAAGCCGGCGAGGAACTTGG
CGTCACCAACAAAGGAAGACTTGTGGCCCGACTCATCCCGGTGCAGGCCGCGGAGCGTTCTCGCGAAGCCCTGATTGAAT
CAGGTGTCCTGATTCCGGCTCGTCGTCCACAAAACCTTCTCGACGTCACCGCCGAACCGGCGCGCGGCCGCAAGCGCACC
CTGTCCGATGTTCTCAACGAAATGCGCGACGAGCAGTGA

Structures

Toxin

• AlphaFold DB: V0SSH7

Antitoxin

• AlphaFold DB: V0YUB5