Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 4132208..4132935 Replicon chromosome
Accession NZ_CP095533
Organism Escherichia coli strain GN02461

Toxin (Protein)


Gene name higB Uniprot ID V0YLE2
Locus tag MW400_RS19345 Protein ID WP_000547555.1
Coordinates 4132624..4132935 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag MW400_RS19340 Protein ID WP_000126294.1
Coordinates 4132208..4132627 (-) Length 140 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MW400_RS19320 (4127293) 4127293..4127820 - 528 WP_001078777.1 electron transport protein HydN -
MW400_RS19325 (4127969) 4127969..4128979 - 1011 WP_029130985.1 DNA-binding transcriptional regulator AscG -
MW400_RS19330 (4129239) 4129239..4130696 + 1458 WP_001107889.1 PTS cellobiose/arbutin/salicin transporter subunit IIBC -
MW400_RS19335 (4130705) 4130705..4132129 + 1425 WP_000110355.1 6-phospho-beta-glucosidase AscB -
MW400_RS19340 (4132208) 4132208..4132627 - 420 WP_000126294.1 helix-turn-helix domain-containing protein Antitoxin
MW400_RS19345 (4132624) 4132624..4132935 - 312 WP_000547555.1 type II toxin-antitoxin system HigB family toxin Toxin
MW400_RS19350 (4133102) 4133102..4133572 - 471 WP_000132967.1 hydrogenase maturation peptidase HycI -
MW400_RS19355 (4133565) 4133565..4133975 - 411 WP_001291918.1 formate hydrogenlyase assembly protein HycH -
MW400_RS19360 (4133972) 4133972..4134739 - 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
MW400_RS19365 (4134739) 4134739..4135281 - 543 WP_000493785.1 formate hydrogenlyase subunit HycF -
MW400_RS19370 (4135291) 4135291..4137000 - 1710 WP_001288122.1 formate hydrogenlyase subunit HycE -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.



Sequences


Toxin        


Download         Length: 104 a.a.        Molecular weight: 12439.18 Da        Isoelectric Point: 9.5146

>T242341 WP_000547555.1 NZ_CP095533:c4132935-4132624 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

>T242341 NZ_OW052570:c1889458-1889033 [Mycobacterium tuberculosis]
GTGACTGACCAGCGCTGGCTGATCGACAAGTCGGCGCTGGTGCGGCTCACGGACAGCCCTGACATGGAAATCTGGTCGAA
CCGGATCGAACGCGGCCTGGTACACATCACGGGCGTGACACGCTTGGAAGTAGGGTTCTCGGCCGAATGCGGGGAGATAG
CGCGACGGGAGTTTCGTGAACCGCCGCTGTCTGCGATGCCCGTGGAATACCTAACCCCGAGAATTGAAGACCGTGCGCTC
GAGGTGCAGACCTTGCTTGCCGACCGCGGACACCACCGTGGCCCGTCGATCCCGGATCTGCTCATCGCCGCGACAGCCGA
ACTGTCGGGCTTGACGGTACTGCACGTCGACAAGGACTTTGACGCCATCGCCGCGCTTACCGGTCAGAAAACAGAACGGC
TCACGCATCGCCCGCCTTCCGCTTAA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15416.42 Da        Isoelectric Point: 4.4596

>AT242341 WP_000126294.1 NZ_CP095533:c4132627-4132208 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp

>AT242341 NZ_OW052570:c1889676-1889455 [Mycobacterium tuberculosis]
ATGAGTGATGTACTGATTCGGGACATCCCCGACGACGTGTTAGCAAGCCTTGACGCGATCGCGGCACGCTTGGGCTTGTC
GCGGACCGAATACATCCGTCGGCGTTTAGCCCAGGATGCGCAGACGGCTCGCGTCACCGTGACAGCCGCGGATCTTCGAC
GCCTCAGGGGTGCGGTTGCCGGTCTGGGCGATCCCGAGCTTATGCGTCAGGCGTGGAGGTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB V0YLE2


Antitoxin

Source ID Structure

References