Detailed information of TA system TA242327

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 2564871..2565242
• Replicon: chromosome
• Accession: NZ_CP095533
• Organism: Escherichia coli strain GN02461

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: F4VC37
• Locus tag: MW400_RS11915
• Protein ID: WP_001317028.1
• Coordinates: 2565048..2565242 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	2564871..2565049 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MW400_RS11890 (2560826)	2560826..2562199	+	1374	WP_000123707.1	ATP-dependent RNA helicase DbpA	-
MW400_RS11895 (2562328)	2562328..2563263	-	936	WP_001157411.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
MW400_RS11900 (2563315)	2563315..2564550	-	1236	WP_000040852.1	site-specific integrase	-
MW400_RS11905 (2564552)	2564552..2564767	-	216	WP_000079604.1	excisionase XisR	-
- (2564871)	2564871..2565049	+	179	NuclAT_0	-	Antitoxin
- (2564871)	2564871..2565049	+	179	NuclAT_0	-	Antitoxin
- (2564871)	2564871..2565049	+	179	NuclAT_0	-	Antitoxin
- (2564871)	2564871..2565049	+	179	NuclAT_0	-	Antitoxin
MW400_RS11910 (2564846)	2564846..2565055	-	210	WP_000276809.1	double-strand break reduction protein RcbA	-
MW400_RS11915 (2565048)	2565048..2565242	-	195	WP_001317028.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
MW400_RS11920 (2565299)	2565299..2566108	-	810	WP_000166319.1	recombination protein RecT	-
MW400_RS11925 (2566101)	2566101..2568701	-	2601	WP_000105135.1	exodeoxyribonuclease VIII	-
MW400_RS11930 (2568803)	2568803..2569078	-	276	WP_000632297.1	protein RacC	-
MW400_RS11935 (2569153)	2569153..2569323	-	171	WP_001352098.1	YdaE family protein	-
MW400_RS11940 (2569323)	2569323..2569544	-	222	WP_000560222.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.89 Da
• Isoelectric Point: 8.9538

>T242327 WP_001317028.1 NZ_CP095533:c2565242-2565048 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T242327 NZ_OW052570:c1410543-1410115 [Mycobacterium tuberculosis]
ATGTTCCTTCTCGACGCCAACGTGCTGCTGGCTGCACACCGCGGTGACCACCCGAATCACCGAACCGTCCGCCCCTGGTT
CGATCGACTGCTCGCGGCTGACGACCCCTTCACAGTGCCGAACCTGGTATGGGCGTCGTTCCTCCGGCTGGCAACGAATC
GACGCATCTTCGAGATTCCGTCACCGCGAGCAGAGGCATTCGCATTCGTCGAAGCCGTCACCGCCCAGCCCCATCACCTT
CCGACGAACCCCGGTCCCAGACACCTGGTGCTGCTGCGAAAACTCTGCGACGAGGCCGACGCATCGGGCGACTTGATACC
TGACGCGGTACTCGCGGCCATAGCAGTGGGGCATCACTGCGCCGTGGTGAGCCTGGACAGGGATTTCGCCCGGTTTGCCT
CGGTGCGCCACATTCGCCCGCCGCTCTAG

Antitoxin

Nucleotide

• Length: 179 bp

>AT242327 NZ_CP095533:2564871-2565049 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A7L6LHV7

Antitoxin