Detailed information of TA system TA242323

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 1319455..1320149
• Replicon: chromosome
• Accession: NZ_CP095533
• Organism: Escherichia coli strain GN02461

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: V0YKG6
• Locus tag: MW400_RS05995
• Protein ID: WP_001263485.1
• Coordinates: 1319751..1320149 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: MW400_RS05990
• Protein ID: WP_000554758.1
• Coordinates: 1319455..1319748 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MW400_RS05970 (1314961)	1314961..1315710	+	750	WP_000093945.1	C40 family peptidase	-
MW400_RS05975 (1315808)	1315808..1317520	-	1713	Protein_1148	flagellar biosynthesis protein FlhA	-
MW400_RS05980 (1317492)	1317492..1318277	+	786	WP_000207541.1	putative lateral flagellar export/assembly protein LafU	-
MW400_RS05985 (1318348)	1318348..1319403	+	1056	WP_001226177.1	DNA polymerase IV	-
MW400_RS05990 (1319455)	1319455..1319748	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
MW400_RS05995 (1319751)	1319751..1320149	+	399	WP_001263485.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
MW400_RS06000 (1320159)	1320159..1320611	+	453	WP_001059900.1	GNAT family N-acetyltransferase	-
MW400_RS06005 (1320930)	1320930..1322069	+	1140	WP_000521554.1	RNA ligase RtcB family protein	-
MW400_RS06010 (1322066)	1322066..1322680	+	615	WP_000602129.1	peptide chain release factor H	-
MW400_RS06015 (1322742)	1322742..1323251	+	510	WP_000469793.1	metal-dependent hydrolase	-
MW400_RS06020 (1323268)	1323268..1323549	-	282	WP_000106438.1	hypothetical protein	-
MW400_RS06025 (1323558)	1323558..1325015	-	1458	WP_001293011.1	cytosol nonspecific dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15418.84 Da
• Isoelectric Point: 7.3840

>T242323 WP_001263485.1 NZ_CP095533:1319751-1320149 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGILPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLCQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T242323 NZ_OW052570:c1015805-1015431 [Mycobacterium tuberculosis]
GTGATCCTGGTCGACACTTCGGTATGGATTGAGCACCTGCGCGCCGCCGACGCGCGACTCGTCGAGCTGCTGGGCGATGA
CGAGGCCGGTTGCCATCCGCTCGTCATCGAGGAGCTGGCGCTTGGCTCGATCAAGCAGCGAGACGTTGTTCTCGATCTGT
TGGCCAACCTCTACCAGTTTCCGGTGGTGACCCACGACGAAGTGTTGCGGCTTGTCGGTCGGCGGCGGTTGTGGGGTCGG
GGACTCGGTGCCGTCGATGCCAACCTTCTTGGTTCGGTGGCTCTGGTTGGCGGCGCGCGACTATGGACGCGGGACAAGCG
GTTGAAGGCGGCGTGCGCGGAAAGCGGTGTTGCGCTGGCTGAGGAAGTGTCCTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT242323 WP_000554758.1 NZ_CP095533:1319455-1319748 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT242323 NZ_OW052570:c1015999-1015802 [Mycobacterium tuberculosis]
ATGAGGACGACGGTGACCGTTGACGACGCCTTGTTAGCCAAAGCGGCCGAATTGACTGGGGTGAAAGAGAAGTCGACGCT
CCTGCGCGAGGGGTTGCAGACACTGGTCCGGGTGGAGAGCGCCCGGCGGTTGGCGGCTCTCGGCGGCACCGACCCGCAAG
CTACCGCGGCGCCGAGACGCCGGACGTCGCCCCGGTGA

Structures

Toxin

• AlphaFold DB: V0YKG6

Antitoxin

• AlphaFold DB: S1QAE3