Detailed information of TA system TA242317

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 8011..8280
• Replicon: plasmid p6156-1
• Accession: NZ_CP095530
• Organism: Escherichia coli strain GN06156

Toxin (Protein)

• Gene name: hok
• Uniprot ID: G9G195
• Locus tag: MW397_RS23275
• Protein ID: WP_001323520.1
• Coordinates: 8164..8280 (+)
• Length: 39 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	8011..8076 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MW397_RS23240 (3785)	3785..4324	+	540	WP_000290813.1	single-stranded DNA-binding protein	-
MW397_RS23245 (4382)	4382..4615	+	234	WP_000005990.1	DUF905 family protein	-
MW397_RS23250 (4679)	4679..6637	+	1959	WP_103930214.1	ParB/RepB/Spo0J family partition protein	-
MW397_RS23255 (6692)	6692..7126	+	435	WP_000845930.1	conjugation system SOS inhibitor PsiB	-
MW397_RS23260 (7123)	7123..7885	+	763	Protein_9	plasmid SOS inhibition protein A	-
MW397_RS23265 (7854)	7854..8042	-	189	WP_001299721.1	hypothetical protein	-
- (8011)	8011..8076	+	66	NuclAT_1	-	-
- (8011)	8011..8076	-	66	NuclAT_0	-	Antitoxin
- (8011)	8011..8076	-	66	NuclAT_0	-	Antitoxin
- (7854)	7854..8078	+	225	NuclAT_0	-	-
- (7854)	7854..8078	+	225	NuclAT_0	-	-
- (7854)	7854..8078	+	225	NuclAT_0	-	-
- (7854)	7854..8078	+	225	NuclAT_0	-	-
- (7854)	7854..8078	-	225	NuclAT_0	-	-
MW397_RS23270 (8064)	8064..8213	+	150	Protein_11	plasmid maintenance protein Mok	-
MW397_RS23275 (8164)	8164..8280	+	117	WP_001323520.1	type I toxin-antitoxin system Hok family toxin	Toxin
MW397_RS23280 (8515)	8515..8874	-	360	WP_077990621.1	hypothetical protein	-
MW397_RS23285 (9175)	9175..9462	+	288	WP_000107542.1	hypothetical protein	-
MW397_RS23290 (9581)	9581..10402	+	822	WP_042021726.1	DUF932 domain-containing protein	-
MW397_RS23295 (10699)	10699..11208	-	510	WP_001595287.1	transglycosylase SLT domain-containing protein	-
MW397_RS23300 (11623)	11623..12006	+	384	WP_000124981.1	conjugal transfer relaxosome DNA-binding protein TraM	-
MW397_RS23305 (12197)	12197..12883	+	687	WP_000332484.1	PAS domain-containing protein	-
MW397_RS23310 (12977)	12977..13204	+	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (1-34)

Sequences

Toxin

Protein

• Length: 39 a.a.
• Molecular weight: 4455.23 Da
• Isoelectric Point: 8.5110

>T242317 WP_001323520.1 NZ_CP095530:8164-8280 [Escherichia coli]
VCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 117 bp

>T242317 NZ_OW052570:c491452-491048 [Mycobacterium tuberculosis]
ATGATCCTCATCGACACATCGGCCTGGGTGGAGTACTTCCGTGCCACCGGATCAATCGCCGCTGTCGAAGTACGCCGGCT
GCTGTCCGAAGAAGCAGCGCGAATCGCTATGTGTGAGCCCATTGCGATGGAAATCTTGAGTGGCGCGCTCGACGACAACA
CCCACACGACGCTAGAGCGGCTCGTGAATGGCTTGCCGTCGTTGAACGTTGATGACGCGATTGACTTTCGTGCTGCCGCG
GGTATCTATCGCGCCGCCCGGCGCGCCGGCGAAACGGTTCGAAGCATCAACGACTGCCTCATAGCGGCGCTCGCGATCCG
CCACGGTGCGCGTATCGTCCACCGTGACGCCGACTTTGATGTGATTGCCCGGATTACCAACCTGCAGGCCGCATCGTTTC
GGTGA

Antitoxin

Nucleotide

• Length: 66 bp

>AT242317 NZ_CP095530:c8076-8011 [Escherichia coli]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Toxin

• AlphaFold DB: G9G195

Antitoxin