Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4179910..4180709 Replicon chromosome
Accession NZ_CP095529
Organism Escherichia coli strain GN06156

Toxin (Protein)


Gene name yhaV Uniprot ID B7NDB6
Locus tag MW397_RS19870 Protein ID WP_000347269.1
Coordinates 4180245..4180709 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID V0YUB5
Locus tag MW397_RS19865 Protein ID WP_001309780.1
Coordinates 4179910..4180245 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MW397_RS19850 (4175695) 4175695..4176465 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
MW397_RS19855 (4176481) 4176481..4177815 - 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
MW397_RS19860 (4178190) 4178190..4179761 + 1572 WP_001273738.1 galactarate dehydratase -
MW397_RS19865 (4179910) 4179910..4180245 + 336 WP_001309780.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
MW397_RS19870 (4180245) 4180245..4180709 + 465 WP_000347269.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
MW397_RS19875 (4180764) 4180764..4181573 - 810 WP_000072171.1 aga operon transcriptional regulator AgaR -
MW397_RS19880 (4181822) 4181822..4183102 + 1281 WP_000681943.1 tagatose-bisphosphate aldolase subunit KbaZ -
MW397_RS19885 (4183125) 4183125..4183598 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
MW397_RS19890 (4183609) 4183609..4184388 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
MW397_RS19895 (4184378) 4184378..4185256 + 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
MW397_RS19900 (4185274) 4185274..4185708 + 435 WP_000948818.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4170668..4180709 10041


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17806.22 Da        Isoelectric Point: 9.6924

>T242315 WP_000347269.1 NZ_CP095529:4180245-4180709 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTREAEETH

Download         Length: 465 bp

>T242315 NZ_OW052570:c297334-297086 [Mycobacterium tuberculosis]
ATGGTGATCGATACCTCTGCGCTGGTCGCGATGCTCAACGATGAACCCGAGGCGCAACGGTTCGAGATAGCCGTGGCAGC
AGACCACGTTTGGCTGATGTCGACGGCGTCATATCCGGAGATGGCGACCGTGATCGAAACACGCTTCGGGGAACCGGGGG
GACGTGAACCCAAGGTCAGCGGCCAGCCTCTCCTCTATAAGGGTGACGATTTCGCATGTATCGATATTCGCGCGGTTCTC
GCCGGCTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12345.94 Da        Isoelectric Point: 4.8616

>AT242315 WP_001309780.1 NZ_CP095529:4179910-4180245 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNT
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT242315 NZ_OW052570:c297546-297334 [Mycobacterium tuberculosis]
ATGGAATTGGCGGCTCGAATGGGCGAGACTTTGACACAAGCGGTCGTAGTTGCAGTGCGGGAGCAACTGGCCCGCCGGGC
CGGGCGCACCAGATCCATTTCGCTACGCGAGGAGTTGGCCGCCATTGGCCGGCGCTGCGCGGCCTTACCGGTGCTCGACA
CCCGAGCCGCGGACACGATTCTCGGCTACGACGAGCGCGGGTTGCCCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829IY86

Antitoxin

Source ID Structure
AlphaFold DB V0YUB5

References