Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2394474..2395036 Replicon chromosome
Accession NZ_CP095529
Organism Escherichia coli strain GN06156

Toxin (Protein)


Gene name higB Uniprot ID L4J948
Locus tag MW397_RS11430 Protein ID WP_000605676.1
Coordinates 2394758..2395036 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag MW397_RS11425 Protein ID WP_000781370.1
Coordinates 2394474..2394758 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MW397_RS11410 (2389656) 2389656..2392703 + 3048 WP_012602782.1 formate dehydrogenase-N subunit alpha -
MW397_RS11415 (2392716) 2392716..2393600 + 885 WP_001240565.1 formate dehydrogenase N subunit beta -
MW397_RS11420 (2393593) 2393593..2394246 + 654 WP_000045646.1 formate dehydrogenase-N subunit gamma -
MW397_RS11425 (2394474) 2394474..2394758 - 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
MW397_RS11430 (2394758) 2394758..2395036 - 279 WP_000605676.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MW397_RS11435 (2395222) 2395222..2396232 - 1011 WP_000642407.1 alcohol dehydrogenase AdhP -
MW397_RS11440 (2396366) 2396366..2398063 - 1698 WP_000433464.1 malate dehydrogenase -
MW397_RS11445 (2398220) 2398220..2398357 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
MW397_RS11450 (2398459) 2398459..2398674 - 216 WP_000495766.1 biofilm-dependent modulation protein -
MW397_RS11455 (2399019) 2399019..2399450 + 432 WP_000152305.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10521.10 Da        Isoelectric Point: 7.3562

>T242303 WP_000605676.1 NZ_CP095529:c2395036-2394758 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T242303 NZ_OW052302:c3883130-3882717 [Mycobacterium tuberculosis]
ATGGTGTTCTGCGTCGACACCAGCGCGTGGCATCACGCGGCGCGGCCGGAAGTTGCGCGCCGATGGTTGGCGGCCTTGTC
CGCGGACCAGATCGGCATCTGCGACCACGTGCGGTTGGAGATCCTGTACTCGGCGAACTCCGCTACCGACTACGACGCGC
TCGCCGACGAACTCGACGGCTTGGCCCGTATACCAGTCGGTGCCGAAACCTTTACGCGCGCATGCCAAGTCCAGCGTGAG
CTTGCCCACGTCGCCGGTCTGCATCACCGCAGCGTGAAGATCGCCGATCTTGTCATCGCCGCGGCGGCCGAACTTTCAGG
CACCATCGTGTGGCATTACGACGAGAACTATGACCGGGTCGCCGCCATCACCGGCCAACCTACGGAGTGGATCGTGCCGC
GCGGGACCCTTTAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT242303 WP_000781370.1 NZ_CP095529:c2394758-2394474 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT242303 NZ_OW052302:c3882678-3882421 [Mycobacterium tuberculosis]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G6T1

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References