Detailed information of TA system TA242290

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1080486..1081136
• Replicon: chromosome
• Accession: NZ_CP095529
• Organism: Escherichia coli strain GN06156

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: MW397_RS05030
• Protein ID: WP_000263532.1
• Coordinates: 1080486..1080827 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: D3GSB3
• Locus tag: MW397_RS05035
• Protein ID: WP_000212551.1
• Coordinates: 1080837..1081136 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MW397_RS05005 (1075521)	1075521..1076621	+	1101	WP_001181876.1	flagellar basal body P-ring protein FlgI	-
MW397_RS05010 (1076621)	1076621..1076920	+	300	WP_000867281.1	rod-binding protein	-
MW397_RS05015 (1077096)	1077096..1078472	+	1377	WP_000367245.1	flagellar hook-associated protein FlgK	-
MW397_RS05020 (1078487)	1078487..1079416	+	930	WP_001266799.1	flagellar hook-associated protein FlgL	-
MW397_RS05025 (1079433)	1079433..1080410	+	978	WP_001195938.1	hypothetical protein	-
MW397_RS05030 (1080486)	1080486..1080827	+	342	WP_000263532.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MW397_RS05035 (1080837)	1080837..1081136	+	300	WP_000212551.1	XRE family transcriptional regulator	Antitoxin
MW397_RS05040 (1081194)	1081194..1082036	-	843	WP_000022366.1	winged helix-turn-helix domain-containing protein	-
MW397_RS05045 (1082522)	1082522..1083436	+	915	WP_000949083.1	lateral flagellin LafA	-
MW397_RS05050 (1083641)	1083641..1084957	+	1317	WP_000609678.1	flagellar filament capping protein FliD	-
MW397_RS05055 (1084980)	1084980..1085372	+	393	WP_000285322.1	flagellar export chaperone FliS	-
MW397_RS05060 (1085377)	1085377..1085688	+	312	WP_000415004.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-107)

Antitoxin

• HigA2-like, helix-turn-helix domain (8-88)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13031.86 Da
• Isoelectric Point: 6.4677

>T242290 WP_000263532.1 NZ_CP095529:1080486-1080827 [Escherichia coli]
MWDVETTETFDNWFDAQTVALKEDLLAAMLILAEYGPQLGRPFADTVNDSQFSNMKELRVQHQGNPVRAFFAFDPARRGI
VLCAGDKTGLNEKKFYRDMIKLADSEYRKHLKK

Nucleotide

• Length: 342 bp

>T242290 NZ_OW052302:3044480-3044908 [Mycobacterium tuberculosis]
ATGCGAGCGCTGTTGGACGTCAACGTGCTACTGGCGCTGTTGGACCGCGATCACGTCGACCACGAGCGCGCCCGAGCGTG
GATCACCGGGCAGATCGAGCGGGGATGGGCTTCCTGTGCGATCACCCAAAACGGGTTCGTCCGCGTCATCAGCCAACCGC
GCTATCCCAGCCCAATCTCGGTAGCCCATGCCATCGACTTGTTGGCTCGTGCGACGCACACGCGCTATCACGAGTTCTGG
TCCTGCACCGTCAGCATTCTCGACTCAAAGGTCATCGATCGTTCCCGCCTGCACAGCCCCAAACAGGTCACCGACGCGTA
CTTGCTGGCCCTCGCGGTCGCTCATGATGGCCGATTTGTCACCTTCGATCAGTCGATTGCGTTGACTGCGGTTCCTGGCG
CAACCAAACAGCACCTGGCAACGCTGTAG

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10976.81 Da
• Isoelectric Point: 9.7665

>AT242290 WP_000212551.1 NZ_CP095529:1080837-1081136 [Escherichia coli]
MATLKELMAKQSPESQARIAAKVEEMRQTIALHQLREELNISQTEMAAAMGIKQPTLAKMEQADNDPRLSTLKRYVAALG
GELSIDVKLPNGKRIAFQI

Nucleotide

• Length: 300 bp

>AT242290 NZ_OW052302:3044234-3044476 [Mycobacterium tuberculosis]
ATGCGCACCACGTTGTCCATCGACGACGATGTATTGCTGGCCGTGAAGGAACGGGCACGCAGGGAGAAGCGCACCGCGGG
TGAAATCCTGTCGGATCTGGCCCGGCAAGCGTTAACCAACCAGAATCCCCAGCCTGCGGCGAGCCAGGAGGACGCCTTTC
ATGGCTTCGAACCCCTGCCTCATCGCGGTGGAGCGGTATCGAACGCGCTGATCGATCGGCTTCGTGACGAGGAGGCCGTG
TGA

Structures

Antitoxin

• AlphaFold DB: D3GSB3