Detailed information of TA system TA242274

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 4897607..4898300
• Replicon: chromosome
• Accession: NZ_CP095526
• Organism: Escherichia coli strain GN05505

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: U9ZN09
• Locus tag: MW395_RS24680
• Protein ID: WP_000415585.1
• Coordinates: 4897607..4897903 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: S1EBV2
• Locus tag: MW395_RS24685
• Protein ID: WP_000650107.1
• Coordinates: 4897905..4898300 (+)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MW395_RS24645 (4892741)	4892741..4893220	+	480	WP_001513449.1	Hcp family type VI secretion system effector	-
MW395_RS24650 (4893223)	4893223..4893933	+	711	WP_001513447.1	hypothetical protein	-
MW395_RS24655 (4893940)	4893940..4894272	+	333	WP_000914691.1	DUF2645 family protein	-
MW395_RS24660 (4894318)	4894318..4895667	-	1350	WP_000673357.1	quorum sensing histidine kinase QseC	-
MW395_RS24665 (4895664)	4895664..4896323	-	660	WP_001221493.1	quorum sensing response regulator transcription factor QseB	-
MW395_RS24670 (4896475)	4896475..4896867	+	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
MW395_RS24675 (4896920)	4896920..4897402	+	483	WP_000183505.1	GyrI-like domain-containing protein	-
MW395_RS24680 (4897607)	4897607..4897903	+	297	WP_000415585.1	type II toxin-antitoxin system toxin MqsR	Toxin
MW395_RS24685 (4897905)	4897905..4898300	+	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	Antitoxin
MW395_RS24690 (4898433)	4898433..4900040	+	1608	WP_001295629.1	ABC transporter substrate-binding protein	-
MW395_RS24695 (4900178)	4900178..4902436	+	2259	WP_001281881.1	DNA topoisomerase IV subunit A	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (2-97)

Antitoxin

• Zinc finger, MqsA-type (3-47)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11261.99 Da
• Isoelectric Point: 8.9070

>T242274 WP_000415585.1 NZ_CP095526:4897607-4897903 [Escherichia coli]
MEKRTPHTRLSQVKKLVNTGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Nucleotide

• Length: 297 bp

>T242274 NZ_OW052302:c1612462-1612055 [Mycobacterium tuberculosis]
GTGAGCACGACGCCGGCCGCCGGAGTGCTCGACACGTCGGTGTTCATCGCGACCGAAAGCGGCCGGCAACTCGACGAGGC
GCTGATCCCCGACCGGGTCGCCACCACCGTCGTCACCCTCGCCGAACTGCGCGTCGGCGTGCTGGCCGCGGCGACGACCG
ACATCCGGGCTCAACGCCTGGCGACCCTGGAATCCGTTGCCGATATGGAAACGTTGCCCGTCGACGACGATGCCGCCCGA
ATGTGGGCCCGATTGCGGATCCATCTTGCCGAGTCCGGTCGCCGGGTGCGGATCAACGACCTGTGGATCGCGGCCGTCGC
GGCATCGCGAGCGCTGCCGGTCATCACCCAGGACGACGACTTCGCCGCCCTCGACGGTGCGGCCAGTGTGGAGATCATTC
GGGTCTGA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14703.10 Da
• Isoelectric Point: 9.2136

>AT242274 WP_000650107.1 NZ_CP095526:4897905-4898300 [Escherichia coli]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Nucleotide

• Length: 396 bp

>AT242274 NZ_OW052302:c1612719-1612459 [Mycobacterium tuberculosis]
ATGTCTGAGGTGGCCTCGCGTGAGCTGCGTAACGATACGGCCGGCGTGCTGCGCCGCGTGCGGGCAGGGGAGGACGTCAC
CATCACCGTCAGCGGCCGTCCGGTCGCGGTGCTTACCCCGGTTCGTCCGCGGCGCCGGCGTTGGCTGAGCAAAACGGAGT
TCCTGTCGCGGTTGCGCGGCGCTCAAGCCGATCCCGGGCTCCGTAACGACCTCGCGGTCCTTGCCGGCGACACGACCGAG
GATCTCGGGCCGATCCGGTGA

Structures

Toxin

• AlphaFold DB: U9ZN09

Antitoxin

• PDB: 3HI2
• PDB: 3GA8
• PDB: 3O9X
• PDB: 2KZ8
• PDB: 3FMY
• PDB: 3GN5
• AlphaFold DB: A0A7U9LU65