Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4788612..4789411 Replicon chromosome
Accession NZ_CP095526
Organism Escherichia coli strain GN05505

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag MW395_RS24140 Protein ID WP_000347273.1
Coordinates 4788612..4789076 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag MW395_RS24145 Protein ID WP_001307405.1
Coordinates 4789076..4789411 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MW395_RS24110 (4783613) 4783613..4784047 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
MW395_RS24115 (4784065) 4784065..4784943 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
MW395_RS24120 (4784933) 4784933..4785712 - 780 WP_000406209.1 PTS N-acetylgalactosamine transporter subunit IIC -
MW395_RS24125 (4785723) 4785723..4786196 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
MW395_RS24130 (4786219) 4786219..4787499 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
MW395_RS24135 (4787748) 4787748..4788557 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
MW395_RS24140 (4788612) 4788612..4789076 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
MW395_RS24145 (4789076) 4789076..4789411 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
MW395_RS24150 (4789560) 4789560..4791131 - 1572 WP_001273753.1 galactarate dehydratase -
MW395_RS24155 (4791506) 4791506..4792840 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
MW395_RS24160 (4792856) 4792856..4793626 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T242272 WP_000347273.1 NZ_CP095526:c4789076-4788612 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T242272 NZ_OW052302:1575217-1575525 [Mycobacterium tuberculosis]
ATGCGGCGCGGTGAATTGTGGTTTGCCGCCACACCTGGTGGTGACAGACCAGTACTTGTCCTTACCAGAGATCCGGTGGC
AGACCGCATCGGCGCGGTCGTTGTGGTGGCCCTAACCCGCACCCGCCGAGGCCTGGTGTCGGAATTGGAGCTCACGGCCG
TCGAAAACCGTGTTCCGAGCGACTGCGTCGTCAACTTCGACAACATTCATACGTTGCCACGCACCGCATTCCGACGCCGC
ATCACCCGGCTGTCCCCGGCCCGCCTGCACGAAGCCTGTCAAACACTCCGGGCGAGCACGGGGTGTTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT242272 WP_001307405.1 NZ_CP095526:c4789411-4789076 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT242272 NZ_OW052302:1574985-1575230 [Mycobacterium tuberculosis]
ATGCTCAGCTTCCGCGCCGACGACCACGACGTCGACCTGGCCGACGCGTGGGCGCGTCGCCTACACATCGGCCGCTCCGA
GCTGCTGCGCGATGCGCTGCGACGTCACTTGGCCGCGCTGGCCGCGGACCAGGATGTCCAGGCCTACACCGAGCGACCGT
TGACGGACGACGAGAACGCACTGGCTGAGATCGCCGACTGGGGGCCGGCGGAGGACTGGGCCGACTGGGCCGATGCGGCG
CGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References