Detailed information of TA system TA242262

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3032089..3032739
• Replicon: chromosome
• Accession: NZ_CP095526
• Organism: Escherichia coli strain GN05505

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: MW395_RS15775
• Protein ID: WP_000263532.1
• Coordinates: 3032398..3032739 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: U9YXQ7
• Locus tag: MW395_RS15770
• Protein ID: WP_000212552.1
• Coordinates: 3032089..3032388 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MW395_RS15745	3027537..3027848	-	312	WP_000415004.1	hypothetical protein	-
MW395_RS15750	3027853..3028245	-	393	WP_000285322.1	flagellar export chaperone FliS	-
MW395_RS15755	3028268..3029584	-	1317	WP_000609678.1	flagellar filament capping protein FliD	-
MW395_RS15760	3029789..3030703	-	915	WP_000949083.1	lateral flagellin LafA	-
MW395_RS15765	3031189..3032031	+	843	WP_000022366.1	winged helix-turn-helix domain-containing protein	-
MW395_RS15770	3032089..3032388	-	300	WP_000212552.1	helix-turn-helix transcriptional regulator	Antitoxin
MW395_RS15775	3032398..3032739	-	342	WP_000263532.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MW395_RS15780	3032815..3033792	-	978	WP_001195938.1	hypothetical protein	-
MW395_RS15785	3033809..3034738	-	930	WP_001266799.1	flagellar hook-associated protein FlgL	-
MW395_RS15790	3034753..3036129	-	1377	WP_000367245.1	flagellar hook-associated protein FlgK	-
MW395_RS15795	3036305..3036604	-	300	WP_000867281.1	rod-binding protein	-
MW395_RS15800	3036604..3037704	-	1101	WP_001181876.1	flagellar basal body P-ring protein FlgI	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-107)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-84)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13031.86 Da
• Isoelectric Point: 6.4677

>T242262 WP_000263532.1 NZ_CP095526:c3032739-3032398 [Escherichia coli]
MWDVETTETFDNWFDAQTVALKEDLLAAMLILAEYGPQLGRPFADTVNDSQFSNMKELRVQHQGNPVRAFFAFDPARRGI
VLCAGDKTGLNEKKFYRDMIKLADSEYRKHLKK

Nucleotide

• Length: 342 bp

>T242262 NZ_OW052302:754663-755064 [Mycobacterium tuberculosis]
ATGATCCTTGTCGACTCCGATGTGCTGATCGCGCATTTGCGGGGTGTCGTTGCTGCTCGCGATTGGCTTGTCAGCGCCCG
CAAGGACGGACCGCTGGCGATCAGCGTGGTGTCCACCGCCGAACTCATCGGCGGAATGCGGACCGCCGAACGGCGCGAGG
TGTGGCGCCTGCTTGCATCGTTTCGGGTACAGCCAGCAACCGAGGTAATCGCACGCCGCGCCGGCGACATGATGCGCCGA
TATCGTCGCAGCCACAACCGGATTGGACTTGGCGACTATCTGATAGCTGCTACCGCCGACGTCCAGGACCTGCAATTGGC
AACCCTCAACGTGTGGCATTTCCCCATGTTCGAGCAGCTGAAACCACCATTTGCGGTGCCGGGGCACCGACCGCGGGCAT
GA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11004.86 Da
• Isoelectric Point: 9.7665

>AT242262 WP_000212552.1 NZ_CP095526:c3032388-3032089 [Escherichia coli]
MATLKELMAKQSPESQVRIAAKVEEMRQTIALHQLREELNISQTEMAAAMGIKQPTLAKMEQADNDPRLSTLKRYVAALG
GELSIDVKLPNGKRIAFQI

Nucleotide

• Length: 300 bp

>AT242262 NZ_OW052302:754409-754666 [Mycobacterium tuberculosis]
ATGAAGCGGACCAACATCTACCTCGACGAGGAGCAGACGGCAAGCCTCGACAAGTTGGCCGCGCAAGAAGGTGTTTCGCG
CGCCGAGCTGATCCGCCTCCTGCTGAACCGAGCCCTCACCACCGCTGGGGACGACCTTGCATCGGACCTGCAGGCTATAA
ACGATTCGTTCGGCACGCTTCGCCACCTGGATCCGCCGGTGCGTCGCTCCGGTGGTCGTGAACAGCACCTTGCCCAGGTG
TGGCGCGCCACCTCATGA

Structures

Antitoxin

• AlphaFold DB: U9YXQ7